基于指纹图谱结合化学计量学的不同产地首乌藤标准汤剂研究  

Study on the Standard Decoction of Polygoni Multiflori Caulis from Different Origins based on Fingerprint and Chemometrics

作　　者：王利伟 何民友 邓淙友 官永河 李国卫 曹斯琼 Wang Liwei;He Minyou;Deng Congyou;Guan Yonghe;Li Guowei;Cao Siqiong(Guangdong Yi Fang Pharmaceutical Co.Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Foshan 528244,China)

机构地区：[1]广东一方制药有限公司/广东省中药配方颗粒企业重点实验室,广东佛山528244

出　　处：《广东化工》2024年第6期127-130,共4页Guangdong Chemical Industry

基　　金：国家工业和信息化部2019年产业技术基础公共服务平台项目(2019-00902-1-2)。

摘　　要：目的:通过指纹图谱结合聚类分析等统计学方法针对首乌藤标准汤剂质量标准,探究不同产地首乌藤标准汤剂的差异成分,为其质量标准制定提供据。方法:首先通过前处理考察得到的最佳指纹图谱制样方法,标定20批首乌藤标准汤剂的指纹图谱共有峰,然后结合《中药色谱指纹图谱相似度评价系统》(2012版)评价各共有峰相似度,最后结合化学模式识别方法,筛选出不同产地的差异性标志物。结果:20批首乌藤标准汤剂含有8个共有峰,其相似度评价值均在0.85以上,其中共指认出4个色谱峰;通过聚类分析和主成分分析将20批样品分为4类,同产地样品为一类;根据偏最小二乘法判别分析(OPLS-DA)分析筛选可知,不同产地的主要差异标志物分别是峰1(2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷)、6、7(大黄素)、8(大黄素甲醚)。结论:本研究所建立的指纹图谱方法及差异性标志物的研究可为首乌藤标准汤剂及其制剂的质量标准的提升提供参考。Objective:To explore the different components of Standard decoction of Shouwuteng(Polygoni Multiflori Caulis)from different producing areas by fingerprint combined with cluster analysis and other statistical methods,so as to provide a basis for the formulation of quality standards.Methods:The common peaks of 20 batches of Wuteng standard decoction were calibrated by the best fingerprint sample preparation method obtained by pre-treatment,and then the similarity of common peaks was evaluated combined with the“Traditional Chinese Medicine chromatographic Fingerprint Similarity Evaluation System”(2012 edition).Finally,the differential markers in different regions were screened by chemical pattern recognition method.Results:20 batches of Shouwu Teng standard decoction contained 8 common peaks,and their similarity evaluation values were all above 0.85.A total of 4 chromatographic peaks were identified.The 20 batches of samples were divided into four groups by Hierachical Cluster Analysis(HCA)and Principal Component Analysis(PCA),and the samples from the same place of origin were in the same group.According to partial least squares discriminant analysis(OPLS-DA)analysis and screening,the main differential markers in different regions were peak 1(2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside),6,7(emodin)and 8(emodin methyl ether).Conclusion:The fingerprint method established in this study and the study of differential markers can provide reference for the improvement of the quality standard of Shenwu Decoction and its preparations.

关 键 词：首乌藤 标准汤剂 指纹图谱 化学计量学 高效液相色谱 

分 类 号：TQ[化学工程]