天麻素对缺血缺氧诱导的小胶质细胞中TLR4表达的影响  

The effects of gastrodin on TLR4 expression in microglia induced by ischemia and hypoxia

作　　者：李顺达 赵永强 杨雪珂 陈澍雨 杨艳 袁云 LI Shunda;ZHAO Yongqiang;YANG Xueke;CHEN Shuyu;YANG Yan;YUAN Yun(Department of Human Anatomy,Histology and Embryology,School of basic medical,Kunming Medical University,Kunming 650500;Department of Blood Transfusion,The First Clinical Medical College of Three Gorges University,Yichang 443003;Department of Human Assisted Reproduction Center,Women and Children Health Care Hospital of Jiangxi Province,Nanchang 330006;C-Luminary Biotechnology Co.,Ltd,Chengdu 611731,China)

机构地区：[1]昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明650500 [2]三峡大学第一临床医学院输血科,宜昌443003 [3]江西省妇幼保健院辅助生殖中心,南昌330006 [4]四川携光生物技术有限公司,成都611731

出　　处：《神经解剖学杂志》2024年第1期50-56,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(31760297)。

摘　　要：目的:探究天麻素(GAS)对缺血缺氧性损伤(HIBD)后小胶质细胞toll样受体4(TLR4)表达的影响。方法:体内创建新生大鼠HIBD模型,将30只3 d龄SD大鼠随机组成3组:假手术组(sham)、HIBD模型组、HIBD模型+GAS干预组(HIBD+G);体外创建BV2细胞氧糖剥夺(OGD)模型,实验随机设置对照组(control)、OGD组、OGD+GAS干预组(OGD+G)。Western Blot和免疫荧光双标染色技术均检测体外各组细胞及体内大鼠模型左侧大脑胼胝体区TLR4的表达。结果:OGD诱导的小胶质细胞中TLR4表达显著增加;GAS干预后TLR4表达显著减少(P<0.05)。结论:GAS激活的小胶质细胞TLR4的表达具有抑制效应,从而对HIBD发挥神经保护作用。Objective:To determine the effect of gastrodin(GAS)on toll-like receptor 4(TLR4)expression in microglia after hypoxic-ischemic brain damage.Methods:Hypoxia-ischemic brain damage(HIBD)model was established in neonatal rat in vivo.Thirty 3 d SD rats of were randomly divided into there groups:Sham group,HIBD model group,HIBD model+gastrodin intervention group(HIBD+G).Oxygen glucose deprivation(OGD)model was established in BV2 cells in vitro,Control group(Control),oxygen glucose deprivation group(OGD),OGD+gastrodin intervention group(OGD+G)were randomly set in vitro.Both Western Blot and immunofluorescence staining techniques were used to detect the expression of TLR4 in cells of each group in vitro and in the left corpus callosum region in vivo.Results:The expression of TLR4 was significantly increased in OGD-induced microglia.After gastrodin intervention,TLR4 expression was decreased significantly(P<0.05).Conclusion:GAS can inhibit the expression of TLR4 in activated microglia and thus play a neuroprotective role in HIBD.

关 键 词：缺血缺氧性脑损伤 天麻素 toll样受体4 氧糖剥夺 小胶质细胞 大鼠 

分 类 号：R285[医药卫生—中药学]