马大杂交相思组培快繁技术研究  

Study on the Tissue Culture and Rapid Propagation Techniques for Acacia mangium x A.auriculiformis

作　　者：林佳佳 LIN Jiajia(Forestry Science and Technology Test Center of Fujian Province,Zhangzhou 363600,Fujian,China)

机构地区：[1]福建省林业科技试验中心,福建漳州363600

出　　处：《安徽林业科技》2024年第1期51-55,共5页Anhui Forestry Science and Technology

基　　金：福建省林业科技项目资金--相思优良无性系选择及其快繁技术研究(编号:闽林文[2022]52号)。

摘　　要：选取马大杂交相思优树的当年生带嫩芽的枝条进行腋芽诱导、增殖、生根等研究,在保持马大杂交相思较高的增殖倍数前提下,缩短增殖周期,提高移栽存活率,繁育出健壮的相思组培苗,为杂交相思快速繁殖和推广奠定良好的基础。研究表明,最适宜的外植体消毒方式为75%酒精浸泡10 s,并用0.1%氯化汞处理10 min,存活率达到83%;最佳诱导培养基为MS+6-BA 0.2 mg/L+蔗糖30 g/L,萌芽率达93.17%;最佳增殖培养基为改良MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+蔗糖30 g/L,增殖倍数可达3.32,增殖周期缩短至25d;最佳生根培养基为MS+IBA 0.5 mg/L+IAA 0.1 mg/L+蔗糖15 g/L,生根率达91.33%;苗木驯化移栽30 d后成活率为97.8%。In this paper,new shoots with buds of fineAcaciamangiumx A.auriculiformistrees were selected to make such tests as axil-lary bud induction,multiplication and rooting to shorten the multiplication cycle,improve the transplanting survival rate and cultivate robust tissue culture plantlets,laying a good foundation for rapid propagation and promotion ofAcaciahybrid while maintaining a high multiplication rate.The results showed that the most suitable disinfection of the explants was 10-second-long soaking in 75%alcohol and 10-min-long treatment in 0.1%mercury chloride,with the survival rate reaching 83%.The best induction medium was MS+6-BA 0.2 mg/L+sucrose 30 g/L,with the germination rate reaching 93.17%.The optimal multiplication medium was MS+6-BA 0.5mg/L+NAA 0.1mg/L+sucrose 30 g/L,with the multiplication times possibly reaching 3.32,and the multiplication cycle shortened to 25d.The optimal rooting medium was MS+IBA 0.5 mg/L+IAA 0.1 mg/L+sucrose 15 g/L,with the rooting rate reaching 91.33%.The sur-vival rate of the transplanting plantlets was 97.8%after 30 days of acclimation.

关 键 词：马大杂交相思 增殖周期 组培快繁 玻璃苗 

分 类 号：S792.99[农业科学—林木遗传育种]