4株滑液囊支原体分离株P80脂蛋白的基因克隆及序列分析  

Gene Cloning and Sequence Analysis of P80 Lipoprotein from Four Strains of Mycoplasma synoviae

作　　者：田兴苗 郭磊 王健霖 戴莎莎 司朵朵 龚振兴 李继东[1] Tian Xingmiao;Guo Lei;Wang Jianlin;Dai Shasha;Si Duoduo;Gong Zhenxing;Li J idong(School of Agriculture,Ningxia University,Yinchuan 750021,China;Ningxia Xiaoming Agriculture and Animal Husbandry Co.,Ltd.,Yinchuan 750011,China)

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]宁夏晓鸣农牧股份有限公司,宁夏银川750011

出　　处：《宁夏大学学报（自然科学版）》2024年第1期60-68,共9页Journal of Ningxia University(Natural Science Edition)

基　　金：宁夏家禽疫病防控与健康养殖创新团队基金资助项目;宁夏大学产教融合研究生联合培养示范基地建设基金资助项目;宁夏银川市校企联合创新专项基金资助项目(2022XQ009)。

摘　　要：为研究滑液囊支原体(Mycoplasma synoviae,MS)P80家族脂蛋白的功能及其异同,通过PCR方法从4株滑液囊支原体分离株中分别扩增P80-1,P80-2,P80-3蛋白的基因序列并进行测序,通过生物信息学方法对P80家族脂蛋白的蛋白序列进行分析,寻找保守结构域、抗原表位,并预测功能.结果表明,P80家族脂蛋白均为碱性亲水性稳定蛋白,在N端具有较强疏水性,只有P80-2蛋白具有跨膜螺旋区;P80家族脂蛋白信号肽的位置与疏水区结果相一致;3条P80蛋白具有12个相同的保守结构域及2~4个不同的结构域;有31~32个抗原表位;二级结构均以α-螺旋和无规则卷曲为主;三级结构预测显示,P80-1蛋白具有ABC转运蛋白的功能,P80-2蛋白为周质结合蛋白Ⅱ型,P80-3蛋白具有细胞黏附功能.研究结果可为MS P80家族脂蛋白功能的深入研究以及MS致病机制、病原诊断和疫苗研制提供参考.To investigate the function and diversity within the P80 lipoprotein family of Mycoplasma synoviae(MS),genes encoding P80-1,P80-2,and P80-3 proteins were amplified from four MS isolates using PCR and sequenced.Bioinformatics methods were applied to analyze the protein sequences of the P80 family lipoproteins,identify their conserved structural domains and antigenic epitopes,and predict their functions.The results indicated that the P80 family lipoproteins were basic,hydrophilic,stable proteins,with a strong hydrophobic region at the N-terminus;only the P80-2 protein had a transmembrane helix region.The positions of the signal peptides and hydrophobic regions of the P80 family lipoproteins are consistent;the three P80 proteins share 12 conserved structural domains and have 2 to 4 distinct structural domains;there are 31 to 32 antigenic epitope sites;their secondary structures are primarily composed ofα-helices and random coils;and the tertiary structure predictions show that the P80-1 protein may function as an ABC transporter,the P80-2 protein as a periplasmic binding protein type II,and the P80-3 protein is associated with cell adhesion functions.These findings provide a reference for further research into the function of the MS P80 family lipoproteins,as well as insights into MS pathogenic mechanisms,pathogen diagnosis,and vaccine development.

关 键 词：滑液囊支原体 P80家族脂蛋白 基因克隆 序列分析 

分 类 号：Q93[生物学—微生物学] R392.11[医药卫生—免疫学]