高温胁迫对大白菜类胡萝卜素的影响  

Effect of High Temperature Stress on Carotenoids of Chinese Cabbage

作　　者：尹莲 孙玉东[1,2] 罗德旭 刘璐[1,2] 张雪莲 白甜 顾妍 王林闯 许文钊[1,2] 赵建锋 YIN Lian;SUN Yu-dong;LUO De-xu;LIU Lu;ZHANG Xue-lian;BAI Tian;GU Yan;WANG Lin-chuang;XU Wen-zhao;ZHAO Jian-feng(Jiangsu Xuhuai Area Huaiyin Institute of Agricultural Sciences,Huai’an 223001,China;Huai’an Key Laboratory for Facility Vegetables,Huai’an 223001,China)

机构地区：[1]江苏徐淮地区淮阴农业科学研究所,江苏淮安223001 [2]淮安市设施蔬菜重点实验室,江苏淮安223001

出　　处：《江西农业学报》2024年第1期42-48,共7页Acta Agriculturae Jiangxi

基　　金：江苏省揭榜挂帅项目(JBGS[2021]073);江苏省农业科技自主创新资金项目[CX(21)2020];江苏省淮安市基础研究计划(指导性)项目(HABZ202327);江苏省淮安市自然科学研究计划联合专项(HABL202229)。

摘　　要：为探讨高温胁迫下大白菜叶片对逆境的响应机制,以叶片颜色和抗逆性存在差异的大白菜品种245、改良青杂3号和紫裔为试验材料,对其幼苗进行了昼/夜温度为40℃/30℃、相对湿度为60%左右的高温胁迫处理,分析了高温胁迫对大白菜幼苗生长及其生理特性的影响。结果表明:胁迫条件下,3个品种大白菜叶片的类胡萝卜素在高温处理下均呈现先下降后上升的变化趋势;随着胁迫时间的增加,品种245在高温处理后第6天类胡萝卜素含量最低(123.51μg/g),在处理后第10天达到顶峰(148.64μg/g)。改良青杂3号和紫裔呈现出不同程度的波动,其中改良青杂3号对热处理更加敏感,在第4天后达到最低值(97.54μg/g);紫裔品种在初始时类胡萝卜素含量差别变化不明显,在热处理后第8天达到最低值(124.50μg/g)。实时荧光定量PCR结果显示,大白菜高温相关基因BraA01g019700.3C和BraA04g000960.3C对热胁迫响应明显,大白菜类胡萝卜素相关基因BraA01g019700.3C、BraA07g002160.3C和BraA04g000960.3C的转录水平均呈先上升后下降的变化趋势;相关性分析结果显示,BraA05g001160.3C(P<0.01;r=0.000)和BraA01g019200.3C(P<0.05;r=0.040)的转录丰度与类胡萝卜素含量显著相关。In order to investigate the response mechanism of Chinese cabbage leaf blades under high temperature stress,Chinese cabbage 245,Gailiang Qingza 3 and Ziyi with different leaf colors and stress resistance were used as experimental materials,and their seedlings were subjected to heat stress at 40℃/30℃and 60%relative humidity.The effects of stress on the growth and physiological characteristics of Chinese cabbage seedlings were researched.the results showed that the carotenoids in leaf blades of three Chinese cabbage varieties showed a trend of first decrease and then increase under heat treatment.With the increase of heat stress duration,the carotenoid content of Chinese cabbage 245 was lowest,with 123.51μg/g at the 6th day,and then peaked to 148.64μg/g at the 10th day.Gailiang Qingza 3 and Ziyi showed different degrees of fluctuation,and Gailiang Qingza 3 was more sensitive to heat treatment,reaching the lowest value of 97.54μg/g at the 4th day.The carotenoid content of Ziyi showed little difference and reached the lowest level of 124.50μg/g at the 8th day under heat treatment.Real-time fluorescence quantitative PCR results showed that BraA01g019700.3C and BraA04g000960.3C in Chinese cabbage were significantly responsive to heat stress.The transcription levels of carotenoids related genes BraA01g019700.3C and BraA04g000960.3C in Chinese cabbage increased first and then decreased.The correlation analysis results showed that the transcription abundance of BraA05g001160.3C(P<0.01,r=0.000)and BraA01g019200.3C(P<0.05,r=0.040)was significantly correlated with carotenoid content.

关 键 词：高温 大白菜 类胡萝卜素 相对表达水平 

分 类 号：S634.1[农业科学—蔬菜学]