复合垂直流人工湿地基质理化性质对nirS和nirK型反硝化菌多样性的影响  

Effects of Substrate Physical and Chemical Properties on the Diversity of nirS and nirK Denitrifying Bacteria in an Integrated Vertical-Flow Constructed Wetland

作　　者：赵伟 朱四喜 夏国栋 高宁 赵斌 王众 ZHAO Wei;ZHU Si-xi;XIA Guo-dong;GAO Ning;ZHAO Bin;WANG Zhong(College of Eco-environment Engineering,Guizhou Minzu University,Guiyang 550025,P.R.China;The Karst Environmental Geological Hazard Prevention Key Laboratory of State Ethnic Affairs Commission,Guiyang 550025,P.R.China;College of Humanities and Science of Guiyang,Guiyang 550025,P.R.China;Qinhuangdao Marine Environmental Monitoring Center Station of The State Oceanic Administration,Qinhuangdao 066000,P.R.China)

机构地区：[1]贵州民族大学生态环境工程学院,贵州贵阳550025 [2]国家民委喀斯特环境地质灾害防治重点实验室,贵州贵阳550025 [3]贵阳人文科技学院,贵州贵阳550025 [4]国家海洋局秦皇岛海洋环境监测中心站,河北秦皇岛066000

出　　处：《水生态学杂志》2024年第2期132-140,共9页Journal of Hydroecology

基　　金：2024年贵州省基础研究(自然科学)计划项目(黔科合基础-ZK[2024]一般490);国家自然科学基金(31560107)。

摘　　要：深入研究复合垂直流人工湿地基质理化性质及其对相关脱氮反硝化微生物的影响,可为解析人工湿地污水处理及提高脱氮效率提供理论依据。2020年12月采集贵州喀斯特地区复合垂直流人工湿地4口池(一级垂直流下行池、一级垂直流上行池、二级垂直流下行池、二级垂直流上行池)的基质样品,用Illumina高通量测序技术获得反硝化功能基因序列,分析其种群组成和多样性特征,并采用冗余分析揭示反硝化菌群落与基质理化性质之间的相关性。结果表明,复合垂直流人工湿地中4口池的基质理化性质存在显著差异;pH在4口池中均呈中性,溶解氧(DO)呈先下降后升高趋势,下行池的基质电导率(EC)均比上行池要高;总氮(TN)、总磷(TP)含量随着人工湿地梯级呈显著下降趋势,未出现厌氧现象;NO_(3)^(-)-N和NH_(4)^(+)-N含量在二级垂直流下行池中大量积累并达到峰值。在多样性分析中,nirS、nirK型反硝化菌丰度随着人工湿地梯级呈先降低后升高的趋势,多样性随人工湿地级数逐渐降低,且下行池丰度多样性显著高于上行池。基质中nirS型反硝化菌共5门9属,nirS型为6门14属,变形菌门是nirS、nirK型反硝化菌群的共同优势菌门,相对丰度为55.36%~65.72%、30.06%~45.66%。nirS、nirK型反硝化菌共有OTUs数分别为392、245,并与反硝化菌群OTUs数目趋势一致,从多角度表明nirS型反硝化菌群丰度明显高于nirK型,且共有OTUs占有率高达41.6%和41.7%,表明各级池中反硝化菌群相似度较高。冗余分析表明基质中nirS和nirK反硝化菌群的构建主要受pH、NH4+-N、DO、TP、TN影响,且nirS型对pH、nirK型对NH4+-N的响应更为强烈。In this study,we explored the effects of substrate physical and chemical properties on the community diversity of nirS and nirK denitrifying bacteria in an integrated vertical-flow constructed wet-land in the karst area of south China.In December 2020,substrate samples were collected in four pools(the first-level vertical flow descending pool,the first-level vertical flow ascending pool,the second-lev-el vertical flow descending pool,and the second-level vertical flow ascending pool)of the integrated ver-tical-flow constructed wetland,and the sequences of the nirS and nirK denitrifying functional bacteria were obtained by Illumina Qualcomm sequencing technique for species composition and community di-versity analysis.The relationship between the denitrifying bacteria and the substrate physical and chemi-cal properties was analyzed by redundancy analysis.Results show that there were significant differences in substrate physical and chemical properties of the four pools.Among them,pH was neutral in all four ponds,DO decreased initially and then increased,and substrate conductivity(EC)of the descending pool was higher than that of the ascending pool.The contents of TN and TP decreased significantly while cas-cading through the constructed wetlands,and there was no anaerobic activity.The contents of NO_(3)^(-)-N and NH_(4)^(+)-N accumulated and peaked in the secondary vertical flow descending pool.The abundance of nirS and nirK denitrifying bacteria decreased initially and then increased in the four cascaded pools,while community diversity of the denitrifying bacteria gradually decreased.Diversity in the descending pools was significantly higher than in the ascending pools.The nirS and nirK denitrifying bacteria identified in the substrate included,respectively,9 genera and 5 phyla,and 14 genera and 6 phyla.Proteus was the common dominant phylum of nirS and nirK denitrifying bacteria,with a relative abundance of 55.36%-65.72%,30.06%-45.66%,respectively.The dominant bacteria genera were Rhodobacter,Dechloromon-as,Rhod

关 键 词：nirS和nirK型 反硝化菌 Illumina高通量测序 冗余分析 复合垂直流人工湿地 

分 类 号：Q178.1[生物学—水生生物学]