平丝变色卧孔菌生物学特性及驯化  

Biological Characteristics and Domestication of Physisporinus lineatus

作　　者：刘珍珍 李守勉[1] 任慧慧 牛彩云 李明[1] 李国杰 LIU Zhenzhen;LI Shoumian;REN Huihui;NIU Caiyun;LI Ming;LI Guojie(College of Horticulture,Key Laboratory of Vegetable Germplasm Innovation and Utilization in Hebei Province,Collaborative Innovation Center for the Vegetable Industry of Hebei,Hebei Agricultural University,Baoding 071001,Hebei,China)

机构地区：[1]河北农业大学园艺学院,河北省蔬菜种质创新与利用重点实验室,河北省蔬菜产业协同创新中心,河北保定071001

出　　处：《食用菌学报》2024年第2期50-63,共14页Acta Edulis Fungi

基　　金：贵州省科技计划项目[黔科合支撑(2019)2451-2];河北农业大学引进人才科研专项(YJ 201849);河北省科技支撑计划资助项目(2053731D);河北省科技计划项目重点研发计划(21326315D)。

摘　　要：平丝变色卧孔菌(Physisporinus lineatus)子实体采自贵州省黔东南苗族侗族自治州黎平县,组织分离得到菌株,采用单因素实验和正交实验研究其生物学特性,并进行驯化。单因素实验结果表明:可溶性淀粉为碳源时平丝变色卧孔菌菌丝生长速度最快,为5.88 mm·d^(-1);酵母浸粉为氮源时菌丝生长速度较快,为5.21 mm·d^(-1);温度为30℃时菌丝生长速度最快,为6.13 mm·d^(-1);在添加CaSO4的培养基中菌丝生长速度最快,为5.25 mm·d^(-1);在C/N为15∶1、45∶1时,菌丝生长速度较快,分别为5.17、5.02 mm·d^(-1);在黑暗条件下培养时菌丝生长速度最快,为5.90 mm·d^(-1)。正交实验结果表明:平丝变色卧孔菌菌丝生长最适碳源、氮源、温度和pH分别为可溶性淀粉、酵母浸粉、30℃、7。首次成功驯化平丝变色卧孔菌,接种92~105 d后收获子实体。研究结果可为平丝变色卧孔菌的开发利用提供参考。A fruiting body of Physiporinus lineatus was collected from Liping County,Qiandongnan Miao and Dong Autonomous Prefecture,Guizhou Province and then subjected to tissue isolation to yield a strain.The strain was studied for biological characteristics through single factor and orthogonal experiments,and then domesticated.The results of the single factor experiments showed that soluble starch was the optimal carbon source for mycelial growth,where the mycelial growth rate reached 5.88 mm·d^(-1).When yeast extract powder was used as the nitrogen source,the mycelial growth rate reached 5.21 mm·d^(-1).For cultivation temperature,mycelial growth rate was the greatest at 30℃,reaching 6.13 mm·d^(-1).Addition of CaSO4 in the cultivation medium promoted mycelial growth rate to 5.25 mm·d^(-1).Preferable C/N ratios were 15∶1 and-1-145∶1,under which the mycelial growth rate reached 5.17 mm·d and 5.02 mm·d respectively.In terms of lighting condition,the mycelial growth rate was the greatest when cultured in the dark,reaching 5.90 mm·d^(-1).The orthogonal experiment showed that the optimal carbon source,nitrogen source,temperature,and pH for mycelial growth were soluble starch,yeast extract powder,30℃,and pH7,respectively.The isolated strain developed fruiting bodies 92–105 d after inoculation,marking the success in the domestication of P.lineatus for the first time.The research provided a reference for the development and utilization of P.lineatus.

关 键 词：变色卧孔菌属 单因素 药用真菌 正交实验 

分 类 号：S646[农业科学—蔬菜学]