鹅细小病毒强毒株的分离鉴定与遗传进化分析  

Isolation and Identification and Genetic Evolution Analysis of the Goose Parvovirus Strong Strain

作　　者：王志强 黄宇翔 邹跃 张红 杨昊天 董佳强 杨坤 WANG Zhiqjang;HUANG Yuxiang;ZOU Yue;ZHANG Hong;ANG Haotian;DONG Jiaqiang;YANG Kun(Heilongjiang Academy of Agricultural Sciences Animal Husbandry and Veterinary Branch,Qiqihar 161005,China;Heilongjiang Province agriculural product processing industry technology collaborative innovation and promotion system,Harbin 150000,China)

机构地区：[1]黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔161005 [2]黑龙江省农产品加工产业技术协同创新推广体系,黑龙江哈尔滨150000

出　　处：《家禽科学》2024年第4期16-21,I0007,共7页Poultry Science

摘　　要：为了了解齐齐哈尔地区鹅细小病毒(GPV)现地强毒株的流行及抗原变异情况,试验从齐齐哈尔龙江县某养殖场采集疑似小鹅瘟病死雏鹅的肝脾病料进行病毒分离培养,对获得的疑似尿囊液进行PCR检测、血凝性检测、动物回归试验及VP3基因序列分析。结果表明,分离到的病毒株能使12日龄鹅胚在144 h内死亡;血凝性检测未检测出血凝价;动物回归试验,可复制该病,致使5日龄雏鹅在96~144 h内全部死亡;VP3基因PCR扩增条带大约为1 600 bp,与目的条带大小相符;对其进行序列分析,判定该分离株为鹅细小病毒毒株,与我室之前分离鉴定的鹅细小病毒HH10强毒株核苷酸同源性为98.17%,与标准B株核苷酸同源性为97.13%。说明鹅细小病毒基因保守。In order to understand the prevalence and antigenic variation of goose parvovirus virulent strains in Qiqihar area,this study collected liver and spleen disease materials of suspected gosling plague dead goslings from a farm in Longjiang County for virus isolation and culture,and the obtained suspected allantoic fluid was detected by PCR,hemagglutination test,animal regression test and VP3 gene genetic variation analysis.The results showed that the isolated virus strain could kill the geese embryos of 12-day-old within 72~96h;there was no hemagglutination value in the hemagglutination test;animal regression experiment could replicate the disease,and all the goslings of 5-day-old died within 96~144h;the size of the PCR amplification band of VP3 gene was about 1600 bp,which was consistent with the size of the target band.After sequencing analysis,the isolated strain was determined to be a GPV strain.The nucleotide homology was 98.17%between it and the GPV HH10 virulent strain isolated and identified by our laboratory before,and 97.13%with the standard B strain,indicating that it was conservative.

关 键 词：鹅细小病毒 现地株分离鉴定 动物回归试验 遗传进化分析 

分 类 号：S858.33[农业科学—临床兽医学]