水稻抗倒伏候选基因的鉴定与功能分析  

Identification and functional analysis of candidate genes for lodging resistance in rice

作　　者：西若曲宗 向登豪 郭子龙 姚义龙 董发明 熊立仲 XIRUO Quzong;XIANG Denghao;GUO Zilong;YAO Yilong;DONG Faming;XIONG Lizhong(National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China;Hubei Hongshan Laboratory,Wuhan 430070,China;Cross Strait Joint Research Institute,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]作物遗传改良全国重点实验室,武汉430070 [2]湖北洪山实验室,武汉430070 [3]福建农林大学海峡联合研究院,福州350002

出　　处：《华中农业大学学报》2024年第2期75-84,共10页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金创新群体项目(31821005)。

摘　　要：为进一步挖掘控制水稻抗倒伏的基因,以524份水稻种质资源为材料,采用GWAS(genome-wide association analysis)鉴定与抗倒伏性状显著关联的位点qRLG7,通过基因表达水平分析和候选基因关联分析确定调控水稻抗倒伏性的候选基因,在分析候选基因的表达特征和启动子区自然变异基础上利用CRISPR-Cas9技术构建2个候选基因的突变体家系,考察转基因材料的抗倒伏性状。结果显示,编码肌醇-1-单磷酸酶的2个串联排列基因LOC_Os07g37220和LOC_Os07g37230在离茎秆基部5 cm节间中的表达量显著高于其余候选基因,而且这2个候选基因的表达量在极端抗倒伏和易倒伏2组材料中存在极显著差异;通过候选基因关联分析发现这2个基因启动子区的SNP与离基部5 cm茎秆直径(CD5)的表型值显著关联,且基于显著关联SNP的2种单倍型的CD5表型值存在显著差异,这2个候选基因的2种单倍型启动子活性检测结果也表明2种单倍型之间存在极显著差异;2个基因的功能缺失突变体植株在基部节间抗折力、茎秆厚度、茎秆直径以及株高和穗质量等性状方面与野生型对照存在显著差异。结果表明,候选基因LOC_Os07g37220和LOC_Os07g37230具有一定的抗倒伏功能。Rice is one of the important food crops.Lodging during the production of rice is a common phenomenon that can have a significant impact on the yield and quality of rice.Therefore,developing rice varieties with lodging-resistance is one of the main goals of rice breeding.524 rice germplasm resources were used to further mine the genes that control lodging resistance in rice.Genome-wide association analysis(GWAS)was used to identify the locus qRLG7 that is significantly associated with lodging resistance.The candidate genes regulating lodging resistance in rice were determined by analyzing the expression level and the association of candidate gene.CRISPR-Cas9 technology was used to construct mutant families of two candidate genes on the basis of analyzing the expression characteristics and natural variations of candidate genes in the promoter region.The lodging resistance of mutant families was examined.The results showed that the expression levels of two tandemly arrayed genes(LOC_Os07g37220 and LOC_Os07g37230),both encoding inositol-1-monophosphatase(IMP),were significantly higher than that of the other candidate genes in the internode at 5 cm away from the bottom of culm.The expression levels of the two IMP genes in the highly lodging resistant germplasms were significantly higher than that in the highly lodging-prone germplasms.The SNPs in the promoter regions of the two genes were significantly associated with culm diameter at 5 cm from the bottom(CD5).The significant SNPs of candidate genes were mainly divided into two haplotypes,and the CD5 values of the two haplotypes were significantly different.The activity of the promoters of two genes had significant differences between the two haplotypes as well.The mutants of the two candidate genes had significantly reduced culm-breaking resistance,culm thickness,culm diameter,plant height,and panicle weight compared to the wild-type rice.It is indicated that the candidate gene LOC_Os07g37220 and LOC_Os07g37230 has a certain lodging resistance.It will provide referen

关 键 词：水稻 抗倒伏 关联分析 候选基因 肌醇-1-单磷酸酶(IMP) 

分 类 号：S511.330[农业科学—作物学]