三角梅褪绿脉带病毒广州分离物基因序列分析  

Gene sequence analysis of Bougainvillea chlorotic vein banding virus isolate from Guangzhou

作　　者：卢咏思 陈华宙 张云霞[3] 饶雪琴 LU Yongsi;CHEN Huazhou;ZHANG Yunxia;RAO Xueqin(College of Plant Protection,South China Agricultural University,Guangzhou 510642,China;Guangdong Province Key Laboratory of Microbial Signals and Disease Control,Guangzhou 510642,China;Innovative Institute for Plant Health,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)

机构地区：[1]华南农业大学植物保护学院,广东广州510642 [2]广东省微生物信号与作物病害重点实验室,广东广州510642 [3]仲恺农业工程学院植物健康新研究院,广东广州510225

出　　处：《仲恺农业工程学院学报》2023年第4期40-44,共5页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广东省现代农业产业技术体系花卉创新团队项目(2022KJ121)。

摘　　要：为了揭示三角梅褪绿脉带病毒(Bougainvillea Chlorotic Vein Banding Virus, BCVBV)在广州发生情况及其系统发育关系,本研究从广东省广州市采集疑似三角梅病样42份,经PCR扩增和序列分析,共获得9个BCVBV广州分离物.对这9个分离物及GenBank中相应分离物进行序列分析,并构建系统发育树.结果表明,BCVBV分离物可划分为2个亚组,BCVBV广州分离物与中国台湾、海南分离物属于同一亚组,亲缘关系较近;巴西分离物属于另一亚组,与广州分离物亲缘关系较远.BCVBV序列同源率分析表明,9个广州分离物基因序列核苷酸和氨基酸序列同源率分别为99%~100%和98.1%~100%,与其他分离物核苷酸和氨基酸序列同源率分别为79.1%~98.1%和89.8%~100%.本研究揭示了BCVBV广州分离物与其他分离物之间的系统发育关系,为三角梅BCVBV的研究和三角梅新品种培育奠定了基础.In order to reveal the occurrence and phylogenetic relationships of Bougainvillea chlorotic vein banding virus(BCVBV)in Guangzhou,42 suspected disease samples were collected from Guangzhou,Guangdong Province,and a total of 9 BCVBV Guangzhou isolates were identified by PCR.The corresponding sequences of 9 Guangzhou isolates and BCVBV isolates in GenBank were analyzed,and a phylogenetic tree was constructed based on 9 BCVBV Guangzhou isolates and other BCVBV isolates in GenBank.The phylogenetic analysis showed that all BCVBV isolates were classified into two subgroups,BCVBV Guangzhou isolates were clustered in the same branch,and shared a closer phylogenetic relationship with the isolates from Taiwan and Hainan.However,the Brazil isolates were grouped in a different branch,which had more distantly related to the BCVBV Guangzhou isolates.The identities of nucleotide and amino acid sequence of 9 BCVBV Guangzhou isolates shared 99%~100%and 98.1%~100%,respectively,while that with other isolates from GenBank showed 79.1%~98.1%and 89.8%~100%,respectively.This study reveals the evolutionary relationship between BCVBV Guangzhou isolates and other isolates,laying the foundation for the study of BCVBV and the breeding of Bougainvillea spectabilis.

关 键 词：三角梅(Bougainvillea spectabilis Willd) 三角梅褪绿脉带病毒 同源率 系统发育 

分 类 号：S436.6[农业科学—农业昆虫与害虫防治]