柠檬香桃挥发油成分分析及杀线虫和抗细菌活性  

Chemical composition,nematocidal and antibacterial activities of volatile oil from Backhousia citriodora

作　　者：黄淑容 杨玉婷 蔡腾 李嘉慧 叶春意 单体江 HUANG Shurong;YANG Yuting;CAI Teng;LI Jiahui;YE Chunyi;SHAN Tijiang(College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学林学与风景园林学院,广东广州510642

出　　处：《仲恺农业工程学院学报》2023年第4期52-58,共7页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广东省林业科技创新项目(2021KJCX002);华南农业大学大学生科技创新训练项目(X202110564187)。

摘　　要：通过气相色谱-质谱联用仪(Gas Chromatograph Mass Spectrometer, GC-MS)分析柠檬香桃(Backhousia citriodora)挥发油的化学组成,采用峰面积归一化法测定各化学成分的相对百分含量;同时测定了挥发油对松材线虫(Bursaphelenchus xylophilus)的杀线活性,并采用多孔板-MTT显色法测定挥发油对4种不同供试细菌的抑制活性.从柠檬香桃挥发油中共鉴定出35种成分,占总相对含量的87.17%,主要成分为香叶醛(Geranial)(34.35%)、橙花醛(Neral)(29.88%)和异香叶醛(Isogeranial)(3.91%),占总相对含量的68.14%,其它成分的相对含量均小于3%;柠檬香桃挥发油对松材线虫表现出有效的杀线虫活性,在24 h、48 h和72 h的LC50值分别为138.30μg/mL、107.83μg/mL和85.56μg/mL;抗细菌活性的结果显示柠檬香桃挥发油对番茄疮痂病菌(Xanthomonas vesicatoria)的抑制活性最好,IC50值为154.11μg/mL,对黄瓜角斑病菌(Pseudomonas lachrymans)、根癌土壤杆菌(Agrobacterium tumefaciens)和枯草芽孢杆菌(Bacillus subtilis)的LC50值均大于200μg/mL.柠檬香桃挥发油对松材线虫和番茄疮痂病菌表现出较好的抑杀活性,本研究为柠檬香桃挥发油资源的开发和利用提供重要的理论依据.In this study,the chemical composition of the volatile oil from Backhousia citriodora was analyzed by gas chromatography-mass spectrometry(GC-MS),and the relative percentage content of each chemical component was determined by peak area normalization method.The nematocidal activity of the volatile oil against Bursaphelenchus xylophilus was determined,and antibacterial activities against Agrobacterium tumefaciens,Pseudomonas lachrymans,Xanthomonas vesicatoria and Bacillus subtili were also determined in sterile 96-well plates using the microculture tetrazolium(MTT)assay.A total of 35 components were identified from the volatile oil of B.citriodorae,which accounted for 87.17%of total content.The major compounds were Geranial(34.35%),Neral(29.88%)and Isogeranal(3.91%),which accounted for 68.13%of the total content.The relative contents of other components were less than 3%.The volatile oil had significant nematocidal activity against B.xylophilus,with LC 50 values of 138.30μg/mL,107.83μg/mL and 85.56μg/mL at 24 h,48 h and 72 h,respectively.The results of antibacterial activity showed the best inhibitory activity against X.vesicatoria,with IC 50 value of 154.11μg/mL,while the LC 50 values were all higher than 200μg/mL against P.lachrymans,A.tumefaciens and B.subtilis.The volatile oil showed good inhibitory activity against B.xylophilus and X.vesicatoria.This study provides an important theoretical basis for the development and utilization of volatile oil of B.citriodora.

关 键 词：柠檬香桃(Backhousia citriodora) 挥发油 化学成分分析 杀线虫活性 抗细菌活性 

分 类 号：S789.9[农业科学—林学]