甘氨酸甜菜碱改性玉米醇溶蛋白负载芸苔素内酯对黄瓜种子的发芽影响  

Effect of brassinolide supported by glycine betaine modified polyamine-zein on the germination of cucumber seeds

作　　者：韩韦 周红军[1,2] 刘俊源 吴伟 周新华[1,2] HAN Wei;ZHOU Hongjun;LIU Junyuan;WU Wei;ZHOU Xinhua(College of Chemical Engineering,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Key Laboratory of Agricultural Green Fine Chemicals of Guangdong Higher Education Institution,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)

机构地区：[1]仲恺农业工程学院化学化工学院,广东广州510225 [2]仲恺农业工程学院广东省普通高校农用绿色精细化学品重点实验室,广东广州510225

出　　处：《仲恺农业工程学院学报》2023年第4期59-66,共8页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广东省国际科技合作项目(20200503);广东省教育厅青年创新人才项目(KA2103F4)。

摘　　要：为提高芸苔素内酯(Brassinolide, BR)的应用稳定性和生物活性,减少使用损失,使其缓慢释放,采用乙二醇二缩水甘油醚(Ethylene glycol diglycidyl ether, EGDE)、乙二胺封端的聚乙烯亚胺(Ethylenediamine-terminated Polyethyleneimine, PEI-EDA)、甘氨酸甜菜碱(Glycine betaine, GB)修饰玉米醇溶蛋白(Zein)作为药物载体(AM-Zein-GB),并用于负载BR(BR@AM-Zein-GB).采用红外光谱、扫描电镜等手段对改性产物结构和形貌进行表征.通过反溶剂法制备了平均粒径为174.19 nm的载药纳米粒子,对BR的包封率和载药量分别为45.59%和17.53μg/mg.研究载药纳米粒子在不同乙醇水含量的缓释量,结果表明,在体积分数60%乙醇水溶液下BR@AM-Zein-GB的缓释量最大.在盐胁迫下(120 mmol/L NaCl),0.100 mg/L-BR@AM-Zein-GB对黄瓜种子浸种的发芽率最好,表明BR@AM-Zein-GB具有稳定的生物活性和可控释放的能力.In order to improve the application stability and biological activity of Brassinolide(BR),reduce its use loss and make it release slowly,in this study,ethylene glycol diglycidyl ether(EGDE),ethylenediamine-terminated Polyethyleneimine(PEI-EDA),and glycine betaine(GB)were used to modify Zein to load BR(BR@AM-Zein-GB).The structure and morphology of the modified products were characterized by Fourier transform infrared spectroscopy(FTIR)and Scanning electron microscopy(SEM).The drug-loaded nanoparticles with an average particle size of 174 nm were prepared by the anti-solvent method.The encapsulation efficiency and drug loading capacity of BR were 45.59%and 17.53μg/mg,respectively.The sustained release of drug-loaded nanoparticles in different ethanol water contents was evaluated.The results showed that the maximum sustained release of BR@AM-Zein-GB was obtained at 60%ethanol aqueous solution by volume.Being soaked in 0.100 mg/L-BR@AM-Zein-GB,the germination of cucumber seed was the best under salt stress(120 mmol/L NaCl).Which showed that BR@AM Zein GB had stable biological activity and controlled release ability.

关 键 词：芸苔素内酯 玉米醇溶蛋白 黄瓜种子 盐胁迫 

分 类 号：TB34[一般工业技术—材料科学与工程]