Oriented Generation of Novel Thermo-Sensitive Genic Male Sterile Lines with Improved Grain Shape and Outcrossing Rate in Early-Season Rice  

作　　者：ZHANG Huali CHEN Junyu LI Ruiqing WANG Huimei DAI Dongqing LIANG Minmin WU Mingyue MA Liangyong 

机构地区：[1]State Key Laboratory of Rice Biology and Breeding/Chinese National Center for Rice Improvement,China National Rice Research Institute,Hangzhou 310006,China

出　　处：《Rice science》2024年第2期129-133,I0001-I0016,共21页水稻科学（英文版）

基　　金：the Natural Science Foundation of Zhejiang Province,China(Grant Nos.LY24C130004,LY22C135104,and LY23C130002);the National Natural Science Foundation of China(Grant No.31501288);the Open Project Program of State Key Laboratory of Rice Biology and Breeding,China(Grant No.20210207);Central Publicinterest Scientific Institution Basal Research Fund,China(Grant No.CPSIBRF-CNRRI-202203).

摘　　要：Two-line hybrid rice with excellent quality is preferred in the Chinese market.However,there is a trade-off between reducing costs for hybrid seed production and lowering the outcrossing rate of the sterile line,which is largely determined by the stigma exsertion rate(SER).In this study,we constructed mutants of male sterility lines with improved grain length(GL)and SER in three elite early-season indica rice varieties through targeted manipulation of the TMS5 and GS3 genes using CRISPR/Cas9-mediated multiplex systems.We obtained a series of marker-free gs3 single mutants and gs3tms5 double mutants with significantly higher SER,longer grains,and increased 1000-grain weight compared with the wild type(WT).Importantly,the typically thermo-sensitive genic male sterile(TGMS)trait with a higher SER was observed in gs3tms5 mutants,and their F1 hybrids exhibited remarkable improvements in grain shape and yield-related traits.Our findings provided an efficient method to generate new valuable TGMS germplasm with improved SER through the mutagenesis of GS3 and TMS5 synergistically,and demonstrated that GS3 had pleiotropic effects on grain size,SER,and grain quality in early-season indica rice.

关 键 词：STERILE ELITE SEASON 

分 类 号：S511.31[农业科学—作物学]