掺钴羟基磷灰石的制备及体外成骨诱导性能研究  

Preparation of cobalt-doped hydroxyapatite and its osteogenic induction properties in vitro

作　　者：张子悦 魏晓博 闫凛 朱苏迪 王成 樊晓霞 ZHANG Ziyue;WEI Xiaobo;YAN Lin;ZHU Sudi;WANG Cheng;FAN Xiaoxia(Medical school of Yan’an University,Yan’an 716000,China)

机构地区：[1]延安大学医学院,陕西延安716000

出　　处：《延安大学学报（自然科学版）》2024年第1期45-50,56,共7页Journal of Yan'an University：Natural Science Edition

基　　金：国家自然科学基金项目(31900965);陕西省科技厅项目(2022JM-547)。

摘　　要：为优化羟基磷灰石(HAp)在治疗骨缺损时的诱导成骨能力,采用化学沉淀法制备一系列不同Co/Ca摩尔比的掺钴羟基磷灰石(Co-HAp),表征其形貌和结构,并研究其体外成骨诱导能力。结果表明,合成的Co-HAp具有典型的磷灰石结构。Co/Ca摩尔比为0.1%时,Co-HAp的结晶度最低。当Co/Ca摩尔比为0.1%,浸提液浓度为12.5μg/mL时,Co-HAp促BMSCs增殖能力最佳,具有良好的细胞相容性。该浓度下的Co-HAp可使BMSCs的ALP活性增强,钙结节增多,表现出较好的体外成骨诱导能力。该研究可为骨缺损修复领域探索更丰富的修复材料提供理论依据。To optimize the bone-inducing ability of hydroxyapatite(HAp)in the treatment of bone defects,a series of cobalt-doped hydroxyapatite(Co-HAp)with different Co/Ca molar ratios were prepared by chemical precipita⁃tion method,its morphology and structure were characterized,and its osteogenic induction ability was studied in vitro.The results showed that the synthesized Co-HAp had a typical apatite structure.When the molar ratio of Co/Ca was 0.1%,the crystallinity of Co-HAp was the lowest.When the molar ratio of Co/Ca was 0.1%and the concentration of the extract was 12.5μg/mL,Co-HAp had the best proliferation ability and good cytocompatibility.At this concentration,Co-HAp could enhance the ALP activity of BMSCs and increase the number of calcium nodules,and show better osteogenic induction ability in vitro.This study can provide theoretical basis for exploring more abundant repair materials in the field of bone defect repair.

关 键 词：掺钴羟基磷灰石 羟基磷灰石 化学沉淀法 细胞增殖 成骨性能 

分 类 号：R318.08[医药卫生—生物医学工程]