Determination of Total Flavonoids in Acanthopanax senticosus(Rupr. et Maxim.) Harms by Enzymolysis and Its Technological Optimization  

作　　者：Li Xinzhi Wang Yudan Wang Meiliang Liu Tianyang Gao Ruihua Qiu Youwen Zhang Shunjie 

机构地区：[1]College of Life Sciences,Northeast Agricultural University,Harbin 150030,China [2]College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,China [3]Mudanjiang Branch of Heilongjiang Forestry Science College,Mudanjiang 157006,Heilongjiang,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2024年第1期53-60,共8页东北农业大学学报（英文版）

基　　金：Supported by the Breeding Techniques for New Varieties of Acanthopanax senticosus(CZKYF2022-1-B023)。

摘　　要：In order to optimize the ultrasonic extraction technique for the total flavonoid of leaf yellows plus, the contents of 21 leaf yellows plus total flavonoid from four regions in Heilongjiang Province were comparatively analyzed. The ultrasonic extraction technology was optimized by Box-Behnken response surface method, and the total flavonoid content of 21 kinds of Acanthopanax senticosus(Rupr. et Maxim.) Harms from different producing areas were analyzed by cluster analysis. The optimal process conditions were determined as ultrasonic time 30 min, solid-liquid ratio 1 : 12 and ultrasonic power 250 W, and the average yield of the total flavonoid was 1.453 mg·g^ (-1). By optimizing the ultrasonic-assisted extraction method, the total flavonoid content from different producing areas was compared in the experiment, which provided certain data support for the optimization of the extraction process in the future and laid a certain theoretical foundation for the quality analysis of Chinese medicinal materials.

关 键 词：Acanthopanax senticosus(Rupr.et Maxim.)Harms total flavonoid Box-Behnken response surface test ultrasonic-assisted extraction 

分 类 号：R283.3[医药卫生—中药学]