性别与生境因素对大林姬鼠肠道菌群多样性的影响  

Effects of Gender and Habitat Factors on Intestinal Flora Diversity of Apodemus peninsulae

作　　者：周宝丽 黄笑然 万祥旭 杨文建 金志民[1] ZHOU Baoli;HUANG Xiaoran;WAN Xiangxu;YANG Wenjian;JIN Zhimin(Mudanjiang Normal University,Mudanjiang,Heilongjiang 157011,China)

机构地区：[1]牡丹江师范学院,黑龙江牡丹江157011

出　　处：《贵州农业科学》2024年第3期97-103,共7页Guizhou Agricultural Sciences

基　　金：牡丹江师范学院生物学学科卓越研究生培养模式改革创新项目(MSYZYXM2022004);牡丹江师范学院生物学学科研究生导学思政团队项目(KCSZTD-2022012)。

摘　　要：【目的】探明不同因素对大林姬鼠肠道菌群结构与多样性的影响,为进一步研究大林姬鼠环境适应性机制奠定理论基础。【方法】采用Illumina高通量测序技术对大林姬鼠盲肠样品16S rRNA V3~V4区进行高通量测序,分析大林姬鼠肠道菌群结构和多样性及性别、生境等因素对大林姬鼠肠道菌群多样性的影响。【结果】大林姬鼠肠道菌群在门水平下,优势菌门为厚壁菌门(Firmicutes,雌66.266%、雄61.866%)和拟杆菌门(Bacteroidetes,雌15.478%、雄12.019%);在属水平下,优势菌属为unclassified Lachnospiraceae(雌24.665%、雄24.714%)、Lachnospiraccac NK4A136 Group(雌19.313%、雄11.612%)和unclassified Muribaculaceae(雌12.962%、雄9.040%)。雌性大林姬鼠样品间肠道菌群结构相似性高,而雄性的差异较大。阔叶林生境的大林姬鼠样品间肠道菌群结构相似性高,针阔混交林的差异较大。【结论】不同性别、生境因素对大林姬鼠肠道菌群结构影响较大。【Objective】The effects of different factors on the structure and diversity of intestinal flora in Apodemus peninsulae were explored to lay the theoretical basis for the further study of environmental adaptability mechanism of A.peninsulae.【Method】The 16S rRNA V3-V4 regions of cecum samples in A.peninsulae were sequenced by Illumina high throughput sequencing technology to analyze the effects of gender,habitat and other factors on intestinal flora structure and diversity of A.peninsulae.【Result】At the phylum level,the dominant bacteria of intestinal flora in A.peninsulae were Firmicutes(female 66.266%,male 61.866%)and Bacteroidetes(female 15.478%,male 12.019%).At the genus level,the dominant bacteria were unclassified Lachnospiraceae(female 24.665%,male 24.714%),Lachnospiraceae NK4A136 Group(female 19.313%,male 11.612%)and unclassified Muribaculaceae(female12.962%,male 9.040%).The intestinal flora structure of female A.peninsulae samples showed high similarity,while the male A.peninsulae samples showed great difference.The intestinal flora structure of A.peninsulae samples in broad-leaved forest was highly similar,while the intestinal flora structure of A.peninsulae samples in coniferous and broad-leaved mixed forest was greatly different.【Conclusion】Different gender and habitat factors had great effects on intestinal flora structure of A.peninsulae.

关 键 词：大林姬鼠 肠道菌群 性别 生境 高通量测序 菌群结构 多样性 

分 类 号：S763.7[农业科学—森林保护学]