基于HPLC-Q-Exactive-MS技术定性分析冠心七味片的化学成分  

Qualitative analysis of chemical constituents of Guanxin Qiwei tablets based on HPLC-Q-Exactive-MS technique

作　　者：刘成东 郭俊英 李君 李雨欣 陆景坤 布仁 郑海英[4] 王跃武 LIU Cheng-dong;GUO Jun-ying;LI Jun;LI Yu-xin;LU Jing-kun;BU Ren;ZHENG Hai-ying;WANG Yue-wu(School of Pharmacy,Inner Mongolia Medical University,Hohhot 010010;Department of Pharmacy,Armed Police General Hospital in Inner Mongolia Autonomous Region,Hohhot 010010;School of Basic Medicine,Inner Mongolia Medical University,Hohhot 010010;Department of Cardiology,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010010)

机构地区：[1]内蒙古医科大学药学院,呼和浩特010010 [2]武警内蒙古自治区总队医院药剂科,呼和浩特010010 [3]内蒙古医科大学基础医学院,呼和浩特010010 [4]内蒙古医科大学附属医院心内科,呼和浩特010010

出　　处：《中南药学》2024年第3期691-698,共8页Central South Pharmacy

基　　金：内蒙古自治区科技计划项目(No.2023YFHH0082);内蒙古医科大学面上项目(No.YKD2024MS015);内蒙古自治区蒙医药协同创新中心成果转化培育项目(No.MYYXTPY202306);内蒙古自然科学基金项目(No.2020MS08045)。

摘　　要：目的采用高效液相色谱-四极杆/静电场轨道阱高分辨质谱(HPLC-Q-Exactive-MS)技术对冠心七味片的主要成分进行快速、全面的定性分析。方法采用Agilent ZORBAX SB-Aq色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇(A)-0.5%甲酸水(B),流速为1.0 mL·min-1,柱温35℃,进样量10μL,质谱采用正、负离子扫描模式,扫描范围m/z 110~1200。基于化合物的精确质荷比、二级质谱裂解碎片,结合自建数据库比对所含成分进行分析鉴定。结果在冠心七味片中共鉴定出67个化合物,包括30个黄酮类、18个有机酸类、8个萜类、5个木脂素类、4个香豆素类、2个其他类化合物。结论该HPLC-Q-Exactive-MS方法能够对冠心七味片化学成分进行全面、快速定性分析,为其质量控制、药效物质基础研究提供基础。Objective To rapidly and comprehensively determine the main components of Guanxin Qiwei tablets by high performance liquid chromatography-quadrupole/electrostatic field orbit-trap high resolution mass spectrometry(HPLC-Q-Exactive-MS).Methods The chromatography was performed on an Agilent ZORBAX SB-Aq column(4.6 mm×150 mm,5μm)with mobile phase consisting of methanol(A)-0.5% formic acid water(B)at 1.0 mL·min-1.The column temperature was 35℃ and the sample size was 10μL.Positive and negative ion scanning modes were used for the mass spectrometry.Scanning range of m/z was 110 to 1200.Based on the precise mass charge ratio and secondary mass spectrometry of the compounds,the analysis and identification of the components were conducted with the self-built database.Results Totally 67 compounds,including 30 flavonoids,18 organic acids,8 terpenoids,5 lignans,4 coumarins and 2 other compounds were identified in Guanxin Qiwei tablets.Conclusion The HPLC-Q-Exactive-MS method can rapidly and comprehensively analyze the chemical components in Guanxin Qiwei tablets,which provides reference for the quality control and research on pharmacodynamic substances.

关 键 词：冠心七味片 化学成分 定性分析 

分 类 号：R284.1[医药卫生—中药学]