基于UPLC-Q-TOF-MS技术建立银花抗病毒口服液的指纹图谱及多指标含量测定  

Fingerprint of Yinhua antiviral oral liquid and multi-index determination content based on UPLC-Q-TOF-MS

作　　者：谭金桃 周思倩[1,3] 方艳 潘吕婷[3] 梁诗瑶 王雅静 吴娟 龙红萍[1] TAN Jin-tao;ZHOU Si-qian;FANG Yan;PAN Lv-ting;LIANG Shi-yao;WANG Ya-jing;WUJuan;LONG Hong-ping(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007;Department of Pharmacy,Hunan Children’s Hospital,Changsha 410007;Hunan University of Chinese Medicine,Changsha 410208)

机构地区：[1]湖南中医药大学第一附属医院,长沙410007 [2]湖南省儿童医院药学部,长沙410007 [3]湖南中医药大学,长沙410208

出　　处：《中南药学》2024年第3期760-766,共7页Central South Pharmacy

基　　金：湖南省卫生健康委科研项目(No.D202313058462);湖南中医药大学“双一流”学科建设项目(No.22JBZ025)。

摘　　要：目的建立银花抗病毒口服液的UPLC-Q-TOF-MS指纹图谱,对其主要化学成分进行分析鉴定,并测定6个化学成分的含量。方法采用UPLC-Q-TOF-MS技术,正、负离子模式,Agilent ZORBAX Eclipse Plus C18(3.0 mm×100 mm,1.8μm)色谱柱,以水相(A,含0.1%甲酸)和有机相乙腈(B)为流动相梯度洗脱,流速为0.4 mL·min^(-1),进样量为1μL。建立12批银花抗病毒口服液的指纹图谱,确定共有峰并进行相似度评价、聚类分析、主成分分析和偏最小二乘判别分析,通过一级、二级质谱及对照品比对鉴别共有峰的化学成分。结果在正、负离子模式下共鉴定了12批银花抗病毒口服液的指纹图谱,确定共有峰32个,指纹图谱相似度均≥0.978,通过聚类分析将样品各分为4类、3类,与主成分分析一致,筛选出汉黄芩苷、千层纸素A苷、连翘酯苷I、异绿原酸B等8个成分是导致不同批次样品差异的标志性化合物。结论所建立的指纹图谱结合多指标含量测定方法稳定、重复性好、特征性强,可为银花抗病毒口服液的质量评价和临床应用提供有效参考。Objective To establish a UPLC-Q-TOF-MS fingerprint of Yinhua antiviral oral liquid identify its main chemical compositions,and to determine the content of 6 chemical compositions.Methods UPLC-Q-TOF-MS was used.Under positive and negative ion modes,an Agilent ZORBAX Eclipse Plus C18(3.0 mm×100 mm,1.8μm)column was used with gradient elution of an aqueous phase(A,containing 0.1% formic acid)and an organic phase acetonitrile(B)as the mobile phase at a flow rate of 0.4 mL·min^(-1) and an injection volume of 1μL.Fingerprints of 12 batches of Yinhua antiviral oral liquid were established and the common peaks were determined.Similarity evaluation,cluster analysis,principal component analysis and partial least squares discriminant analysis were performed.The chemical components of common peaks were identify through primary and secondary mass spectrometry,and compared with reference materials.Results Totally 32 peaks were identified in the fingerprint spectra of 12 batches of Yinhua antiviral oral liquid under positive and negative ion modes.The fingerprint similarity was≥0.978.The samples were divided into categories four or three by cluster analysis,which was consistent with principal component analysis.Eight components including wogonside,oroxyloside,forsythoside I,and isochlorogenic acid B were screened out as the hallmark compounds for the differences between various batches of samples.Conclusion The fingerprint combined with multi-index content determination method established in this study is stable,repeatable and specific,which can provide reference for the quality evaluation and clinical application of Yinhua antiviral oral liquid.

关 键 词：银花抗病毒口服液 UPLC-Q-TOF-MS 指纹图谱 成分鉴定 含量测定 

分 类 号：R284.1[医药卫生—中药学]