GC-ECD法测定人参归脾丸中17种有机氯类农药残留  

Determination of 17 organochlorine pesticide residues in Renshen Guipi Pills by GC-ECD

作　　者：李正刚 赵磊[1,2] 李文君 张进[3] 郭晶[3] 马彧 LI Zheng-gang;ZHAO Lei;LI Weng-jun;ZHANG Jin;GUO Jing;MA Yu(Department of Traditional Chinese Medicine,Siping Institute for Food and Drug Control,Siping,Jilin 136000,China;不详)

机构地区：[1]四平市食品药品检验所中药室,吉林四平136000 [2]长春中医药大学药学院 [3]通化市食品药品检验所

出　　处：《中国卫生工程学》2024年第1期42-44,48,共4页Chinese Journal of Public Health Engineering

基　　金：吉林省科技厅项目重点研发医药健康领域(20210204151YY)。

摘　　要：目的 建立气相色谱-电子捕获检测法(GC-ECD)同时测定人参归脾丸中17种有机氯农药残留量。方法 人参归脾丸样品经水、丙酮、适量氯化钠超声分散,二氯甲烷萃取,离心转移,有机相用无水硫酸钠脱水后减压浓缩至近干,正己烷溶解,硫酸溶液和水除去杂质,采用GC-ECD法测定样品中17种有机氯类农药残留。结果 17种有机氯类农药在测定范围0.76~100.30 ng/ml内线性关系良好,线性相关系数(r)均大于0.995,检出限(LOD)为0.001 1~0.001 8 mg/kg,平均回收率为73.6%~110.1%。结论 该方法灵敏度高,准确可靠,可用来测定人参归脾丸中17种有机氯类农药残留量。Objective To establish a method of simultaneous determination 17 organochlorine pesticide residues in Rensh⁃en Guipi pills by gas chromatography electron capture detection(GC-ECD).Methods The samples were dispersion with water,acetone and sodium chloride of appropriate amount by ultrasound;The pesticides were extracted with dichlorometh⁃ane,centrifuged migrate.The organic phase was steamed to dry by reducing the pressure after dehydration with anhydrous so⁃dium sulfate.Dissolveed by N-hexane,sulfuric acid solution and water to remove impurities,the pesticides were determined by GC-ECD.Results All the 17organochlorine pesticides showed good linearities in the reasonable rang of 0.76 to 100.30 ng/ml with the linear collection coefficients(r)greater than 0.995.The LODs of each pesticide were all in the range of 0.0011-0.0018 mg/kg,and the average recoveries of all the pesticides were in the range of 73.6%-110.1%.Conclusion This method is highly sensitive,accurate and reliable and can be used to determine 17 organochlorine pesticide residues in Renshen Guipi pills.

关 键 词：人参归脾丸 气相色谱法 有机氯 农药残留 

分 类 号：R9170657.7[医药卫生—药物分析学]