济南市出血热疫区鼠类携带汉坦病毒基因特征分析  

Genetic characteristics of hantavirus in rodents in hemorrhagic fever epidemic area in Jinan City

作　　者：赵红[1] 赵宝添 刘子薇 张钦钰 王蕾 焦海涛[1] ZHAO Hong;ZHAO Baotian;LIU Ziwei;ZHANG Qinyu;WANG Lei;JIAO Haitao(Jinan Municipal Center for Disease Control and Prevention,Jinan,Shandong 250021,China)

机构地区：[1]济南市疾病预防控制中心,山东济南250021

出　　处：《中国预防医学杂志》2024年第1期92-96,共5页Chinese Preventive Medicine

基　　金：山东省医药卫生科技发展计划(2019WS071);济南市卫生健康委员会科技计划项目(2023-公-27)。

摘　　要：目的分析济南市肾综合征出血热(hemorrhagic fever with renal syndrome, HFRS)汉坦病毒(Hantanvirus,HV)基因型别及基因组特征。方法 对2021年济南市汉坦病毒阳性鼠肺组织样本进行病毒分离,利用二代测序技术获得汉坦病毒全基因组序列。应用BioEdit、MEGA 11等软件对基因组序列进行遗传进化、同源比对分析,建立系统进化树,并对核苷酸及氨基酸序列突变进行分析。结果 获得2株济南市汉坦病毒全基因组序列,基因组全长11 944 bp,均为汉城型。济南市汉坦病毒汉城型分离株属于S3亚型分支,济南市汉坦病毒病毒分离株S基因与北京市2005年SEO分离株氨基酸序列同源性最近98.3%~98.6%。结论 利用二代测序方法获得的济南市汉坦病毒全基因组序列,氨基酸序列分析表明其与我国汉坦病毒汉城型相近,与疫苗株相比核苷酸和氨基酸序列突变有明显变异,需密切关注其致病力、毒力及传播力的改变。Objective e To analyze the genotypes and genomic characteristics of hantavirus(HV)causing hemorrhagic fever with renal syndrome(HFRS)in Jinan City.Methods The whole genome sequence of HV was obtained using the second-generation sequencing technology from the lung tissue samples of HV-positive mice in Jinan City in 2021.BioEdit and MEGA 1l were used to conduct genetic evolution and homologous alignment analysis of the genome sequences,plot phylogenetic tree,and analyze nucleotide and amino acid sequence mutations.Results The whole genome sequence of two HV strains was obtained,with a total length of the genome being 11944 bp,both of which were of Seoul virus.The Seoul HV isolates from Jinan belonged to the S3 subtype,and had high level of amino acid homology in its S gene with the isolates from Beijing in 2005(98.3%-98.6%).Conclusions The whole genome sequence of HV isolates from Jinan was obtained using the second-generation sequencing method.Amino acid sequence analysis showed high homology to the Seoul type found in other areas in China,and had obvious mutations in nucleotide and amino acid sequences compared with the vaccine strains.Therefore,it is necessary to pay close attention to changes in its pathogenicity,virulence and transmission.

关 键 词：肾综合征出血热 汉坦病毒 基因型别 进化分析 

分 类 号：R512.8[医药卫生—内科学]