雷斯青霉F-1发酵培养基优化及对枸杞木虱的毒力测定  

Optimization of the culturing medium for Penicillium raistrickii F-1 and determination of its virulence against Bactericera gobica.

作　　者：黎秋雨 柴军发 蒲艳丽 马文田 张晓莉 王芳[2] 贾彦霞[1] LI Qiuyu;CHAI Junfa;PU Yanli;MA Wentian;ZHANG Xiaoli;WANG Fang;JIA Yanxia(School of Agriculture,Ningxia University,Yinchuan 750021,China;Institute of Plant Protection,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002,China)

机构地区：[1]宁夏大学农学院,银川750021 [2]宁夏农林科学院植物保护研究所,银川750002

出　　处：《生态学杂志》2024年第3期815-822,共8页Chinese Journal of Ecology

基　　金：宁夏回族自治区农业科技自主创新资金“十四五”农业高质量发展和生态保护科技创新示范项目(NGSB-2021-10-04)资助

摘　　要：雷斯青霉是本课题组在罹病木虱虫体上分离获得的具有生防效果的菌株,研究提高雷斯青霉的生长性能对进一步提高防治效果具有重要意义。本试验以产孢量为指标,采用单因素试验设计对发酵培养基中不同成分及其浓度进行筛选与优化,并对枸杞木虱三龄若虫进行毒力测定。结果表明,优化后的培养基配方为可溶性淀粉40 g·L^(-1)、酵母粉1 g·L^(-1)、K2-HPO41 g·L^(-1)、MgSO4·7H2O 1.5 g·L^(-1)、KCl 0.5 g·L^(-1)、FeSO40.01 g·L^(-1),产孢量较基础培养基提高了9.84倍。毒力测定结果表明,优化培养基对枸杞木虱三龄若虫在第7天的校正死亡率较同时间基础培养基增加了25.56%。综合产孢量与毒力结果,优化后培养基效果比基础培养基的效果更佳,为提高雷斯青霉防控枸杞木虱的防治效果奠定了基础。Penicillium raistrickii is a strain with biological control effect,which was isolated from the body of the infected Bactericera gobica by our research group.Enhancing the growth performance of P.raistrickii has a great significance to improve its control effect.In this study,a single-factor experiment was conducted to select and optimize different medium components and their concentrations for culturing P.raistrickii using spore production as an indicator.We determined the virulence of P.raistrickii culturing broth against the third instar of B.gobica.The results showed that the optimized medium was composed of 40 g·L^(-1) soluble starch,1 g·L^(-1) yeast powder,1 g·L^(-1) K2HPO4,1.5 g·L^(-1) MgSO4·7H2O,0.5 g·L^(-1) KCl and 0.01 g·L^(-1) FeSO4,which increased spore production by 9.84 times compared to the basic medium.The results of the toxicity assay showed that the corrected mortality rate of the third instar of B.gobica treated by the P.raistrickii culturing broth with the optimized medium at 7 d increased by 25.56%compared to the basal medium.Based on the spore production and virulence results,the optimized medium is more effective than the basic medium in culturing P.raistrickii and controlling B.gobica,which provides a foundation for improving the biological control effect of P.raistrickii against B.gobica.

关 键 词：雷斯青霉 培养基优化 毒力 枸杞木虱 生物防治 

分 类 号：S435.671[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]