基于SCF/c-kit信号通路探讨理气通便方对气滞型慢传输型便秘大鼠肠道动力的影响  

Effect of Liqi Tongbian Formula on Intestinal Motility in Slow Transit Constipation Rats with Qi Stagnation Syndrome Based on SCF/c-kit Signaling Pathway

作　　者：柯丹枫 刘启鸿[2,3] 骆云丰 柯晓[2,3] 胡露楠 严锦贤[4] 任彦 方文怡 赵培琳 KE Danfeng;LIU Qihong;LUO Yunfeng;KE Xiao;HU Lunan;YAN Jinxian;REN Yan;FANG Wenyi;ZHAO Peilin(China-Taiwan Strait(Fujian)Cell Biotechnology Co.,Ltd.,Fuzhou,Fujian 350100,China;The Second Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350003,China;Fujian Provincial Clinical Medical Research Centre of Chinese Medicine for Spleen and Stomach,Fuzhou,Fujian 350003,China;Fujian Provincial Key Laboratory of Chinese Medicinal Preparation for Medical Institutions,Fuzhou,Fujian 350003,China)

机构地区：[1]中海峡(福建)细胞生物科技有限公司,福建福州350100 [2]福建中医药大学附属第二人民医院,福建福州350003 [3]福建省中医脾胃临床医学研究中心,福建福州350003 [4]福建省医疗机构中药制剂重点实验室,福建福州350003

出　　处：《福建中医药》2024年第2期12-16,共5页Fujian Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82274282);福建省自然科学基金项目(2021J01878);中央引导地方科技发展资金项目(2023L3013)。

摘　　要：目的 基于SCF/c-kit信号通路探讨理气通便方对气滞型慢传输型便秘(STC)大鼠肠道动力的影响。方法 将36只Wistar雌性大鼠按随机数字表法分为对照组6只和造模组30只。造模组采用“洛哌丁胺混悬液+夹尾刺激”复制气滞型STC大鼠模型,连续刺激14 d。当大鼠表现出激惹、易怒、烦躁等情况,正常喂养饲料时出现大便干硬、排便数量减少等表现时,说明气滞型便秘模型造模成功。将造模成功的大鼠按照随机数字表法分为模型组、西药组、低剂量组、中剂量组和高剂量组各6只,低、中、高剂量组分别按5.15、10.3、20.6 g/(kg·d)给予理气通便方药液灌胃;西药组按0.18 mg/(kg·d)给予琥珀酸普芦卡必利片混悬液灌胃;对照组和模型组按10 mL/(kg·d)给予无菌水灌胃,每日1次,连续灌胃14 d。比较6组末次给药后6 h的粪便排出量、粪便含水量和小肠推进率;HE染色观察结肠组织病理变化;免疫组化检测结肠组织c-kit蛋白表达水平;Western blot检测结肠组织c-kit、SCF蛋白表达量。结果 HE染色显示:对照组大鼠结肠黏膜完整,杯状细胞和腺体排列整齐,未见炎症细胞聚集;模型组结肠黏膜出现肠腺排列欠整齐,黏膜下层间质血管扩张;各给药组结肠黏膜肠腺排列整齐,未观察到明显上皮损伤。与对照组比较,模型组粪便排出量、粪便含水量和小肠推进率均明显降低(P<0.05),结肠组织c-kit蛋白表达水平和c-kit、SCF蛋白表达量均明显降低(P<0.05)。与模型组比较,西药组、中剂量组、高剂量组粪便排出量、粪便含水率和小肠推进率均明显提高(P<0.05),低剂量组粪便排出量和粪便含水率均明显提高(P<0.05);给药组结肠组织c-kit蛋白表达水平均明显提高(P<0.05);低、中、高剂量组结肠组织c-kit蛋白表达量均明显提高(P<0.05),高剂量组SCF蛋白表达量明显提高(P<0.05)。结论 理气通便方可提高气滞型STC模型大鼠结肠组织中ICCObjective:To investigate the effect of Liqi Tongbian formula on intestinal motility in slow transit constipation(STC)rats with Qi stagnation syndrome based on SCF/c-kit signaling pathway.Methods:Thirty-six female Wistar rats were divided into control group(n=6)and modeling group(n=30)using the random number table method.In the modeling group,"loperamide suspension+tail pinch stimulation"was used to replicate the STC rats with Qi stagnation syndrome model for 14 days.Successfully modeled rats were randomly divided into model group,western medicine group,low-dose group,medium-dose group,and high-dose group,with 6 rats in each group.the low-,medium-,and high-dose groups were administered with Liqi Tongbian formula solution by gavage at doses of 5.15,10.3,and 20.6 g/(kg·d),respectively;the western medicine group was administered with Prucalopride succinate suspension by gavage at doses of 0.18 mg/(kg·d);the control and model groups were given sterile water by gavage at doses of 10 mL/(kg·d),once a day,continuously gavage for 14 days.The quantity of fecal excretion,fecal water content,and small intestine propulsion rate were detected 6 hours after the last administration;the pathological changes of colon tissue was observed by HE staining;the tyrosine kinase growth factor receptor(c-kit)protein expression level of colon tissue was measured by immunohistochemistry;the c-kit and stem cell factor(SCF)protein expression of colon tissue was measured by Western blot.Results:HE staining showed that the colonic mucosa of rats in the control group was intact,with well-arranged cup cells and glands,and no aggregation of inflammatory cells was observed;in the model group,the colonic mucosa appeared to have poorly arranged intestinal glands,and the submucosal mesenchymal vasculature was dilated;in each of the administered groups,the intestinal glands of the colonic mucosa were well-arranged,and no obvious epithelial damage was observed.Compared with the control group,the quantity of fecal excretion,fecal water content,and smal

关 键 词：慢传输型便秘 气滞 理气通便方 ICC SCF/c-kit信号通路 

分 类 号：R285.5[医药卫生—中药学]