UHPLC-MS/MS同时测定清感童饮中6种有效成分在大鼠体内血药浓度及药代动力学研究  

Simultaneous determination of six active ingredient in Qinggan Tongyin by UHPLC-MS/MS and pharmacokinetics in rats

作　　者：祁纪鸽 张孝莹 王丽雯 周昆[1,2] 张玥[1,2] QI Jige;ZHANG Xiaoying;WANG Liwen;ZHOU Kun;ZHANG Yue(Institute of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301607,China;State Key Laboratory for Component-based Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学中医药研究院,天津301617 [2]天津中医药大学组分中药国家重点实验室,天津301617

出　　处：《天津中医药》2024年第4期503-509,共7页Tianjin Journal of Traditional Chinese Medicine

基　　金：天津市中医药重点领域科研项目(2021004)。

摘　　要：[目的]本实验旨在研究清感童饮活性成分在大鼠血浆中的药代动力学特征。[方法]实验建立大鼠血浆中甘草次酸、木犀草素、芹菜素、芍药苷、连翘酯苷A、连翘苷等6种活性成分的超高效液相色谱-串联质谱(UHPLC-MS/MS)检测方法,考察大鼠灌胃给药清感童饮后,6种成分在大鼠体内的药代动力学特征。采用WELCN Ultimate XB-C_(18)色谱柱(2.1 mm×100 mm,3μm)分离;流动相为0.1%甲酸水(A)-乙腈(B);梯度洗脱;进样量3μL;流速为0.2 mL/min;柱温40℃;在多反应监测模式(MRM)下正、负离子同时测定。[结果]结果显示,大鼠血浆样品中各成分的线性关系均良好(r>0.99),其日内、日间精密度、准确度、稳定性、基质效应以及提取回收率均符合生物样品的分析要求。药动学参数结果显示,各成分的t_(1/2z)为1.18~34.12 h,T_(max)为0.44~19.33 h,C_(max)为9.00~318.09μg/L,AUC_(0~t)为12.40~15 969.32 h·μg/L,MRT_(0~t)为2.23~23.19 h。[结论]本实验所建立的检测方法快速、准确、重复性好,可适用于清感童饮中甘草次酸、木犀草素、芹菜素、芍药苷、连翘酯苷A、连翘苷在大鼠血浆中的药动学研究。[Objective]The aim of this experiment is to study the pharmacokinetic characteristics of the active ingredients of Qinggan Tongyin in rat plasma.[Methods]An ultra performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)method was established to detect six active ingredients in rat plasma,including glycyrrhetinci acid,luteolin,apigenin,paeoniflorin,forsythiaside A,and phillyrin and investigate the pharmacokinetic characteristics of six components in rats after oral administration of Qinggan Tongyin.Using WELCN Ultimate XB-C_(18) column(2.1 mm×100 mm,3μm)Separation;The mobile phase is 0.1%formic acid water(A)-acetonitrile(B);Gradient elution;Injection volume 3μL;The flow rate is 0.2 mL/min;column temperature is 40℃;Simultaneous determination of positive and negative ions in multi reaction monitoring mode(MRM).[Results]The research results showed that the linear relationships of various components in rat plasma samples were good(r>0.99),and its intra-day and inter-day precision,accuracy,stability,matrix effect and extraction recovery rate all met the analysis requirements of biological samples.The pharmacokinetic parameter results show that the t_(1/2z) of each component is 1.18 to 34.12 h,T_(max) is 0.44 to 19.33 h,C_(max) is 9.00 to 318.09μg/L,AUC_(0~t) is 12.40 to 15969.32(h·μg)/L,and MRT_(0~t) is 2.23 to 23.19 h.[Conclusion]The detection method established in this experiment is fast,accurate,and has good repeatability,and can be applied to the pharmacokinetic study of glycyrrhetinci acid,luteolin,apigenin,paeoniflorin,forsythiaside A,and phillyrin in rat plasma.

关 键 词：清感童饮 药代动力学 UHPLC-MS/MS 血浆 

分 类 号：R284[医药卫生—中药学]