基于高世代姊妹系发掘玉米穗长性状QTL及候选基因  

QTL Mapping and Candidate Genes Analysis for Ear Length in Maize Population Derived from High-Generation Sister Lines

作　　者：孙瑞东 李艺萌 黄然 刘颖[1] 周筱航 丁明阳 刘新航 吕香玲[1] 李凤海[1] SUN Ruidong;LI Yimeng;HUANG Ran;LIU Ying;ZHOU Xiaohang;DING Mingyang;LIU Xinhang;LYU Xiangling;LI Fenghai(College of Agriculture,Shenyang Agricultural University,Shenyang 110866)

机构地区：[1]沈阳农业大学农学院,沈阳110866

出　　处：《植物遗传资源学报》2024年第2期270-278,共9页Journal of Plant Genetic Resources

基　　金：沈阳市科学技术计划种业创新专项(21-110-3-16,21-110-3-09-03,22-318-2-01-02);辽宁中部生态区早熟宜机收玉米新品种培育(2022JH1/10200001)。

摘　　要：玉米穗长通过影响穗粒数,进而影响产量,是育种改良的重要目标性状之一。发掘与穗长性状相关QTL和基因对穗长性状的遗传改良具有重要意义。本研究以一对穗长具有显著差异的高世代姊妹系为亲本,构建F2群体并自交得到F2:3,利用Maize6H-60K基因芯片分别对亲本及F2群体进行基因型分析,并通过ICIM、GCIM和dQTGseq 3种方法对F2和F2:3群体的穗长进行QTL定位。结果表明,3种方法共定位到7个与穗长相关QTL位点和43个QTNs,解释了2.04%~10.24%的表型变异。交叉分析不同方法得出的定位结果,在6号染色体上发现一个调控穗长的稳定QTL qEL6.01。根据定位区间内基因注释的结果,并结合参考基因的表达谱数据,共筛选出5个在雌穗中表达的候选基因:Zm00001d035514、Zm00001d035526、Zm00001d035537、Zm00001d035553和Zm00001d035535。本研究结果为玉米穗长基因的克隆及育种改良奠定了基础。The ear length,which associates to the number of grains per ear and the yield production,is one of the important traits in maize breeding.It is of great significance to explore QTL and genes related to ear length traits in its genetic improvement.In this study,two high-generation sister lines showing significant difference on ear length were used to generate a F2 population and derived F2:3 population.Both parents and the F2 population lines were genotyped using the Maize6H-60K Gene Chip.The ear length in the F2 and F2:3 populations were measured and subjected for QTL mapping by three statistical methods ICIM,GCIM and dQTG-seq.The results showed that 7 QTLs and 43 QTNs related to ear length were identified by three methods,each of which explained the phenotypic variation of 2.04%-10.24%.A stable QTL qEL6.01 on chromosome 6 was found by different methods.According to the results of gene annotation in the qEL6.01 and the expression profile data of reference genes,five candidate genes Zm00001d035514,Zm00001d035526,Zm00001d035537,Zm00001d035553 and Zm00001d035535 were proposed associating with ear length.Collectively,these results laid a foundation for the future gene isolation and their use in ear length improvement in maize.

关 键 词：玉米 穗长性状 QTL 基因芯片 候选基因 

分 类 号：S513[农业科学—作物学]