华南籼稻骨干亲本稻瘟病基因检测与抗性评价  

Detection and Evaluation of Blast Resistance Genes in Backbone Indica Rice Parents from South China

作　　者：陈睿[1] 陈子强[1] 凌波 农雯 田大刚[1] 陈建民[1] CHEN Rui;CHEN Ziqiang;LING Bo;NONG Wen;TIAN Dagang;CHEN Jianmin(Biotechnology Institute of Fujian Academy of Agricultural Sciences,Fuzhou 350003)

机构地区：[1]福建省农业科学院生物技术研究所,福州350003

出　　处：《植物遗传资源学报》2024年第3期386-398,共13页Journal of Plant Genetic Resources

基　　金：福建省属公益类科研院所基本科研专项(2021R1027009,2022R1027003)。

摘　　要：选用Pi2、Piz-t、Pi9、Pi25、Pi5、Pita、Pia、Ptr、Pi1、Pikm、Pi54等11个抗稻瘟病基因的功能性分子标记,对华南稻区近年育成的90个籼稻骨干亲本进行抗性基因鉴定与稻瘟病抗性评价。结果表明,综合抗性等级达到高抗、抗、中抗、中感、感及高感的亲本分别为0份、3份、35份、38份、14份和0份,且年度间表现较为一致。不同鉴定时期的稻瘟病抗性级别相关性分析进一步显示,苗瘟与叶瘟、苗瘟与穗颈瘟以及叶瘟与穗颈瘟的抗性级别呈极显著正相关。对上述11个抗性基因在90个亲本中的分布进行检测,发现除Pi9基因以外,其他10个抗性基因在亲本中的分布频率分别为75.56%(Pi54)、70.0%(Pi5)、47.78%(Pi2)、31.11%(Pi25和Pia)、20.0%(Ptr)、15.56%(Pi1)、13.32%(Pita)、4.44%(Pikm)和1.11%(Piz-t)。11个抗性基因在不同省际间育成亲本中的分布频率差异较大。进一步结果表明,随着聚合抗性基因数量的增加,亲本抗性水平呈相应提升趋势,但不同抗性基因组合对于提升水稻亲本抗病性的贡献呈现明显差异。本研究为华南稻区新育成籼型常规水稻品种的合理布局及抗稻瘟病基因的育种应用提供有益的参考。By deployment of 11 functional markers of blast resistance genes,including Pi2,Piz-t,Pi9,Pi25,Pi5,Pita,Pia,Ptr,Pi1,Pikm,and Pi54,this study analyzed the resistance gene diversity in the newly-developed 90 indica rice varieties from south China.The results showed that 0,3,35,38,14,and 0 varieties exhibited high-resistance,resistance,moderate resistance,moderate susceptibility,susceptibility,and high-susceptibility,respectively.The results tested for blast resistance were highly correlated in years.By analyzing the correlation among different detection stages,significant positive correlations in seedling blast and leaf blast,seedling blast and neck blast,as well as leaf blast and neck blast were observed.Except Pi9,the frequency of other 10 resistance genes in population were 75.56%(Pi54),70.0%(Pi5),47.78%(Pi2),31.11%(Pi25 and Pia),20.0%(Ptr),15.56%(Pi1),13.32%(Pita),4.44%(Pikm)and 1.11%(Piz-t),respectively.Moreover,the distribution frequency of 11 resistance genes varied significantly among parents from different provinces.Further results also indicated that as the number of resistance genes increased,the level of resistance of parents showed a corresponding upward trend.Furthermore,the contribution of different resistance gene combinations to improve the disease resistance of rice parents showed significant differences.The current study provided a basis for the rational distribution of resistant conventional indica rice varieties with different genotypes in South China.

关 键 词：籼稻 稻瘟病 基因 抗性 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]