无鱼粉饲料中补充限制性氨基酸对鲤抗氧化及部分免疫酶活性的影响  

Effect of restrictive amino acid supplementation in fishmeal-free diets on antioxidant and some immune enzyme activities in common carp (Cyprinus carpio)

作　　者：李卓 彭祖想 任同军 韩雨哲[1,2,3] LI Zhuo;PENG Zuxiang;REN Tongjun;HAN Yuzhe(College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;Dalian Ocean University,Laboratory of Aquatic Animal Nutrition and Feed,Dalian 116023,China;Key Laboratory of Fish Applied Biology and Aquaculture in North China,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023 [2]大连海洋大学水产动物营养与饲料实验室,辽宁大连116023 [3]大连海洋大学辽宁省北方鱼类应用生物学与增养殖重点实验室,辽宁大连116023

出　　处：《河北渔业》2024年第3期5-10,25,共7页

基　　金：辽宁省教育厅青年科技人才“育苗”项目(QL202004);大连海洋大学“蔚蓝英才”人才计划项目。

摘　　要：为探讨在无鱼粉饲料中补充限制性氨基酸对鲤(Cyprinus carpio)抗氧化性能和免疫酶活性的影响,研究设计了5组等氮等脂的饲料,分别添加不同水平的限制性氨基酸(Met∶Lys=0.318),即0%(G1组)、0.5%(G2组)、1.0%(G3组)、1.5%(G4组)、2.0%(G5组)。选取初始体质量为(55.54±0.09)g的鲤150尾,进行了为期8周的养殖试验。研究结果显示,适量补充限制性氨基酸能够提高鲤的总抗氧化能力(T-AOC)、总超氧物歧化酶(T-SOD)和过氧化氢酶(CAT)活性;同时降低丙二醛(MDA)含量。G4和G5组的T-AOC活性显著高于对照组(P<0.05),G2、G3、G4和G5组的T-SOD和CAT活性显著高于对照组(P<0.05),G5组的MDA含量显著低于G1、G2和G3组(P<0.05)。随着限制性氨基酸补充水平的增加,鲤肝脏的ACP、AKP和LZM活性也呈上升趋势。G3、G4和G5组的ACP活性显著高于对照组(P<0.05),G4和G5组的AKP和LZM活性显著高于对照组(P<0.05)。根据试验结果,可以得出结论:在本试验条件下,适量补充限制性氨基酸能够增强鲤的抗氧化性能和部分免疫酶活性,其中2.0%的限制性氨基酸补充量效果最好。In order to investigate the impact of restrictive amino acid supplementation in fishmeal-free diets on the antioxidant properties and immune enzyme activity of common carp(Cyprinus carpio),five groups of isonitrogenous and isolipid feeds were prepared,with varying levels of restrictive amino acid(Met:Lys=0.318) supplementation:0%(group G1),0.5%(group G2),1.0%(group G3),1.5%(group G4),and 2.0%(group G5).In total,150fish with an initial body weight of(55.54± 0.09)g were selected for an 8-week culture trial.The results indicated that fishmeal-free diets supplemented with appropriate amounts of restrictive amino acids increased the total antioxidant capacity(T-AOC),total superoxide dismutase(T-SOD),and catalase(CAT)activities,while decreasing the malondialdehyde(MDA)content in the carp.CAT activity was significantly higher in groups G2,G3,G4,and G5compared to the control group(P<0.05),and the MDA content was significantly lower in group G5compared to groups G1,G2,and G3(P<0.05).The activity of acid phosphatase(ACP),alkaline phosphatase(AKP),and lysozyme(LZM)in the carp liver increased with higher levels of restrictive amino acid supplementation.The ACP activity in groups G3,G4,and G5was significantly higher than that in the control group(P<0.05),and the AKP and LZM activities in groups G4and G5were significantly higher than those in the control group(P<0.05).In conclusion,under the experimental conditions of this study,fishmeal-free diets supplemented with appropriate amounts of restrictive amino acids improved the antioxidant performance and some immune enzyme activities in common carp,with the best results observed with 2.0%restrictive amino acid supplementation.

关 键 词：无鱼粉 限制性氨基酸 鲤(Cyprinus carpio) 抗氧化 非特异性免疫 

分 类 号：S963[农业科学—水产养殖]