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作 者:李江维 韩晓光 张荣坤 孙承鑫 LI Jiangwei;HAN Xiaoguang;ZHANG Rongkun;SUN Chengxin(Liaoning Tanghe Reservoir Administration Bureau Co.,LTD.,Liaoyang 111000,China;Liaoning Water Resources Management Group Co.,Ltd.,Shenyang 110000,China;Dalian Ocean University,Dalian 116023,China)
机构地区:[1]辽宁省汤河水库管理局有限责任公司,辽宁辽阳111000 [2]辽宁省水资源管理集团有限责任公司,辽宁沈阳110000 [3]大连海洋大学,辽宁大连116023
出 处:《河北渔业》2024年第3期16-25,共10页
摘 要:针对采捕的野生亚洲公鱼(Hypomesus transpacificus nipponensis)雄亲鱼精液量少,传统上在亚洲公鱼发眼卵阶段即放流成活率低的特点,改进了繁殖方法,采用“湿法授精+孵化篓孵化”方式,2023年3月在辽宁省辽阳汤河水库进行了亚洲公鱼人工增殖改进试验。3月19日至21日在汤河水库上游东河头采集亚洲公鱼亲鱼200 kg,获受精卵3 000万粒,受精率达83.4%,孵化率达65.4%。4月6日向水库投放1 637.6万尾仔鱼。胚胎与仔鱼显微观察:受精卵弱黏性,有黏丝。水温(10±2)℃,其胚胎发育历时429 h,孵化积温4 290℃·h。卵黄囊平均体积与日龄相关方程:V_(卵黄囊体积)=0.121 4-0.020 6d+0.000 9d~2(R~2=0.995 8)。此改进方法延长生长阶段至水花鱼苗阶段放流,提高了放流效率。Male parents of wild Hypomesus transpacificus nipponensis have less semen and the low survival rate of the eye egg stage of H.transpacificus nipponensis,the reproduction method was improved,the “wet method of insemination + incubation basket incubation” method was adopted.In March 2023,an artificial proliferation improvement experiment of H.transpacificus nipponensis was conducted in Tanghe Reservoir,Liaoyang,Liaoning Province.From March 19 to 21,200 kg of H.transpacificus nipponensis were collected from the upstream of Tanghe Reservoir,and 30 million fertilized eggs were obtained,with fertilization rate of 83.4% and hatching rate of 65.4%.On April 6,16.376 million larvae were released into the reservoir.Microscopic observation of embryo and larvae showed that,the fertilized eggs were weakly sticky and had sticky filaments.At the water temperature of(10 ±2)℃,the embryo was developed for 429 h,and the incubation product temperature was 4 290 ℃·h.Equation for average yolk sac volume and day age correlation was V_(yolksac volume) =0.121 4-0.020 6d+0.000 9d~2(R~2=0.995 8).This improved method extended the growth stage to the fry stage,and improved the release efficiency.
关 键 词:亚洲公鱼(Hypomesus transpacificus nipponensis) 人工繁殖 胚胎发育 仔鱼发育
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