基于GEO数据库光损伤性皮肤病的生物信息学分析  

Bioinformatics Analysis of Photo-damaged Dermatosis Based on GEO Database

作　　者：董俊娟 都日娜 刘贺磊 赵鹏伟[1] 高耀星 DONG Junjuan;Durina;LIU Helei;ZHAO Pengwei;GAO Yaoxing(Inner Mongolia Medical University,Hohhot 010059 China;Department of Dermatology,International Mongolian Hospital,Hohhot 010050 China)

机构地区：[1]内蒙古医科大学,呼和浩特010059 [2]内蒙古自治区国际蒙医医院皮肤科,呼和浩特010050

出　　处：《内蒙古医学杂志》2024年第1期11-17,共7页Inner Mongolia Medical Journal

摘　　要：目的通过对紫外线照射后人皮肤表皮细胞基因芯片的生物信息学分析,获得光损伤性皮肤病的关键基因,探索其发病机制。方法从GEO数据库中获取2个紫外线照射后人皮肤表皮细胞株HaCat的基因芯片数据集,第一个基因芯片数据集采用R软件筛选出显著性差异表达基因(DEGs),进行GO分析和KEGG通路分析,另一个基因芯片数据集也采用同样的方法筛选出DEGs,将两者DEGs取交集,进行GO分析和KEGG通路分析,最后构建蛋白质-蛋白质相互作用网络(PPI),通过筛选,获得紫外线损伤HaCat的关键基因。结果GSE198792筛选获得的DEGs有486个(上调246个、下调240个),GSE138800筛选获得的DEGs有90个(上调33个、下调57个)。两者取交集筛选获得20个基因。GO分析显示这些差异表达基因主要与蛋白质转运、表皮生长因子受体信号通路的调控、I型干扰素信号通路的负调控以及对病毒的防御有关,KEGG通路主要与磷酸戊糖途径相关。筛选出了ISG15、IFI6、TKT、LAP3、SAMHD1、BAG3、ALDOC 7个关键基因。结论通过生物信息学分析筛选出紫外线对人皮肤表皮细胞作用的关键基因,预测其在紫外线损伤皮肤中具有重要作用,研究为防治光损伤性皮肤病提供了新思路。Objective Through the bioinformatics analysis of human skin epidermal cell gene microarray after ultraviolet irradiation,the key genes of photo-damaged dermatosis were obtained and its pathogenesis was explored.Methods Two gene chip data sets of human skin epidermal cell line HaCat exposed to ultraviolet rays were obtained from GEO database.The first gene chip data set was screened for significant differentially expressed genes(DEGs)by R software for GO analysis and KEGG pathway analysis.The other gene chip data set was also screened for DEGs by the same method.A GO and KEGG pathway analysis was conducted at the junction of the two DEGs,with a protein-protein interaction network(PPI)then constructed to acquire essential genes.Results There were 486 DEGs obtained by GSE198792 screening(246 up-regulated and 240 down-regulated)and 90 DEGs obtained by GSE138800 screening(33 up-regulated and 57 down-regulated).20 genes were obtained by intersection screening.GO analysis showed that these differentially expressed genes were mainly related to protein transport,regulation of epidermal growth factor receptor signaling pathway,negative regulation of type Ⅰ interferon signaling pathway and defense against viruses,and KEGG pathway was mainly related to pentose phosphate pathway.Seven key genes were identified:ISG15,IFI6,TKT,LAP3,SAMHD1,BAG3 and ALDOC.Conclusion Through bioinformatics analysis,the key genes of UV action on human skin epidermal cells were screened,and it was predicted that they may play an important role in skin injury caused by UV,which provides a new idea for the prevention and treatment of skin diseases caused by UV.

关 键 词：紫外线 皮肤表皮细胞 差异表达基因 生物信息分析 

分 类 号：R758.1[医药卫生—皮肤病学与性病学]