制何首乌不同提取物抗斑马鱼骨质疏松作用研究  

Study on anti-osteoporosis effect of different extracts of Polygoni Multiflori Radix Praeparata on zebrafish

作　　者：高媛媛 阎芸芸 陈卓然 李霄[1] Gao Yuanyuan;Yan Yunyun;Chen Zhuoran;Li Xiao(Faculty of Environment and Life,Beijing University of Technology,Beijing 100124,China)

机构地区：[1]北京工业大学环境与生命学部,北京100124

出　　处：《国际中医中药杂志》2024年第3期315-322,共8页International Journal of Traditional Chinese Medicine

基　　金：北京市教委科技发展基金项目(JC015001201201)。

摘　　要：目的研究制何首乌不同提取物抗斑马鱼骨质疏松作用。方法制备制何首乌、蒽醌和二苯乙烯苷部位和除蒽醌和二苯乙烯苷部位3种样品,应用泼尼松龙构建斑马鱼幼鱼骨质疏松症模型,设置正常对照组、模型组、依替膦酸二钠组和制何首乌不同提取物低、中、高剂量组,采用茜素红染色法考察各组斑马幼鱼头骨矿化面积和骨密度;采用碱性磷酸酶(AKP)和抗酒石酸酸性磷酸酶(TRACP)试剂盒检测斑马幼鱼中成骨细胞和破骨细胞酶活力;采用qRT-PCR法检测各组斑马鱼骨质疏松相关基因Runx2b、col1a2、sparc、vdrb mRNA表达情况。结果与模型组比较,制何首乌不同提取物剂量组头骨矿化面积和骨密度升高(P<0.01);制何首乌、蒽醌和二苯乙烯苷部位和除蒽醌和二苯乙烯苷部位(中、高剂量)组斑马鱼AKP活力升高(P<0.01),TRACP活力降低(P<0.01);制何首乌不同提取物组斑马鱼幼鱼Runx2b、col1a2、sparc、vdrb mRNA水平升高(P<0.01)。结论制何首乌、蒽醌和二苯乙烯苷部位和除蒽醌和二苯乙烯苷部位均具有较好的抗骨质疏松作用,提示何首乌中除蒽醌和二苯乙烯苷类成分是抗骨质疏松主要药效成分外,还有其他化学成分具有抗骨质疏松的效果。Objective To study on anti-osteoporosis effect of different extracts of Polygoni Multiflori Radix Praeparata on zebrafish.Methods Three kinds extracts of Polygoni Multiflori Radix Praeparata,anthraquinone and stilbene glycosides and the removal of anthraquinone and stilbene glycosides were prepared.Prednisolone was used to construct the osteoporosis model of young zebrafish.Normal control group,model group,disodium etidronate group and low-,medium-and high-dosage groups of different extracts of Polygoni Multiflori Radix Praeparata were set up.Alizarin red staining was used to investigate the mineralized skull area and bone density of juvenile zebrafish in each group.Alkaline phosphatase(AKP)and tartrate-resistant acid phosphatase(TRACP)kits were used to detect the activity of osteoblast and osteoclast enzymes in zebra larvae.The qRT PCR method was used to detect the mRNA expressions of osteoporosis related genes Runx2b,col1a2,sparc,and vdrb in each group of zebrafish.Results Compared with model group,the skull mineralized area and bone mineral density in different extracts of Polygoni Multiflori Radix Praeparata significantly increased(P<0.01).Polygoni Multiflori Radix Praeparata,anthraquinone and stilbene glycosides and the removal of anthraquinone and stilbene glycosides(medium-and high-dosage)could significantly increase the AKP activity of zebrafish(P<0.01),and lower the TRAP activity of zebrafish(P<0.01);the mRNA expression levels of Runx2b,col1a2,sparc and vdrb in juvenile zebrafish osteoporosis model were significantly up-regulated by different extracts of Polygoni Multiflori Radix Praeparata.(P<0.01).Conclusion Polygoni Multiflori Radix Praeparata,Anthraquinone and stilbene glycosides and the removal of anthraquinone and stilbene glycosides show better anti-osteoporosis effects.The comparison of the efficacy of three extracts from Polygonum multiflorum shows that in addition to anthraquinone and stilbene glycosides,other chemical components of Polygonum multiflorum have anti-osteoporosis effects.

关 键 词：制何首乌 斑马鱼 骨质疏松 泼尼松龙 

分 类 号：R285.5[医药卫生—中药学]