复方甘乐注射液对小鼠血糖的影响研究  

Study on the Effect of Compound Ganle Injection on Blood Glucose in Mice

作　　者：王丹 林鹏 刘蓉 刘芳 张鹏[1] 袁昕蓉[1] WANG Dan;LIN Peng;LIU Rong;LIU Fang;ZHANG Peng;YUAN Xinrong(Shenyang Institute for Food and Drug Control,Shenyang 110122,China;Liaoning Institute for Drug Control,Shenyang 110036,China)

机构地区：[1]沈阳市食品药品检验所,辽宁沈阳110122 [2]辽宁省药品检验检测院,辽宁沈阳110036

出　　处：《品牌与标准化》2024年第2期21-23,27,共4页

摘　　要：目的:研究复方甘乐注射液对小鼠血糖的影响。方法:选健康雄性KM小鼠70只,随机分为7组,分别为复方甘乐注射液高剂量组(416 mg/kg)、中剂量组(208 mg/kg)、低剂量组(104 mg/kg)、左卡尼汀注射液组(200 mg/kg)、甘乐注射液剂量组(8 mg/kg)、胰岛素注射液组(0.8 U/kg)及空白对照组(氯化钠注射液)。皮下给予相应受试物7天,比较给药后的血糖值。建立高血糖小鼠模型,将90只糖尿病模型小鼠随机分为9组,分别为复方甘乐注射液高剂量组(416 mg/kg)、中剂量组(208 mg/kg)、低剂量组(104 mg/kg),左卡尼汀注射液高剂量组(400 mg/kg)、低剂量组(200 mg/kg),甘乐注射液高剂量组(16 mg/kg)、低剂量组(8 mg/kg),胰岛素注射液组(0.8 U/kg)及模型对照组。同时取10只正常小鼠作为空白对照组(氯化钠注射液)。皮下给予相应受试物7天,比较给药后的血糖值。建立高血糖小鼠模型,将80只糖尿病模型小鼠随机分为8组,分别为复方甘乐注射液组(208 mg/kg)、复方甘乐注射液(208 mg/kg)+胰岛素注射液组(0.4 U/kg)、左卡尼汀注射液组(200 mg/kg)、左卡尼汀注射液(200 mg/kg)+胰岛素注射液组(0.4 U/kg)、甘乐注射液组(8 mg/kg)、甘乐注射液(8 mg/kg)+胰岛素注射液组(0.4 U/kg)、胰岛素注射液组(0.4 U/kg)及模型对照组,同时取10只正常小鼠作为空白对照组(氯化钠注射液)。皮下给予相应受试物7天,比较给药后的血糖值。结果:复方甘乐注射液各剂量组对正常小鼠血糖无显著影响。高血糖模型中,复方甘乐注射液中剂量组(208 mg/kg)、左卡尼汀注射液组(200 mg/kg)、甘乐注射液组(8 mg/kg)及胰岛素注射液组(0.8 U/kg)与模型组比较,差别有统计学意义(P<0.05)。复方甘乐注射液(208 mg/kg)+胰岛素注射液组(0.4 U/kg)、左卡尼汀注射液(200 mg/kg)+胰岛素注射液组(0.4 U/kg)、甘乐注射液(8 mg/kg)+胰岛素注射液组(0.4 U/kg)与模型组比较,差别有统计学意义(P<0.01);并且复方甘�Objective:To study the effect of compound Ganle injection on blood glucose in mice.Method:Seventy healthy male KM mice were selected and randomly divided into seven groups:high-dose group(416 mg/kg),medium dose group(208 mg/kg),low-dose group(104 mg/kg),levocarnitine injection group(200 mg/kg),Ganle injection dose group(8mg/kg),insulin injection group(0.8 U/kg),and blank control group(sodium chloride injection).Subcutaneously administer the corresponding test substance for 7 days and compare the blood glucose levels after administration.To establish a hyperglycemic mouse model,90 diabetes model mice were randomly divided into 9 groups:high dose group(416 mg/kg),medium dose group(208 mg/kg),low dose group(104 mg/kg),high dose group(400 mg/kg),low dose group(200 mg/kg),high dose group(16 mg/kg),low dose group(8 mg/kg)Insulin injection group(0.8 U/kg)and model control group.Simultaneously,10 normal mice were taken as the blank control group(sodium chloride injection).Subcutaneously administer the corresponding test substance for 7 days and compare the blood glucose levels after administration.To establish a hyperglycemic mouse model,80 diabetes model mice were randomly divided into 8 groups:Compound Ganle injection Study on the Effect of Compound Ganle Injection on Blood Glucose in Mice group(208 mg/kg),Compound Ganle injection group(208 mg/kg)+insulin injection group(0.4 U/kg),L-carnitine injection group(200 mg/kg),L-carnitine injection group(200 mg/kg)+insulin injection group(0.4 U/kg),Ganle injection group(8 mg/kg)Ganle injection(8 mg/kg)+insulin injection group(0.4 U/kg),insulin injection group(0.4 U/kg)and model control group,while taking 10 normal mice as blank control group(sodium chloride injection).Subcutaneously administer the corresponding test substance for 7 days and compare the blood glucose levels after administration.Result:Each dose group of Compound Ganle Injection had no significant effect on blood glucose levels in normal mice.In the hyperglycemic model,there was a significant difference(P<0.05)b

关 键 词：复方甘乐 小鼠 四氧嘧啶 

分 类 号：R285.5[医药卫生—中药学]