铁皮石斛组培苗生长影响因子研究  

Study on influencing factors of tissue culture seedling growth of Dendrobium officinale

作　　者：李得萍 陈一鸣 何远秦 董洪雨 易元惠 刘艳军 Li Deping;Chen Yiming;He Yuanqin;Dong Hongyu;Yi Yuanhui;Liu Yanjun(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300392,China)

机构地区：[1]天津农学院园艺园林学院,天津300392

出　　处：《天津农学院学报》2024年第1期18-23,30,共7页Journal of Tianjin Agricultural University

摘　　要：以铁皮石斛组培苗为材料,研究了铁皮石斛组培苗生长的影响因子,以便优化铁皮石斛组培快繁体系。试验结果表明:低盐培养基1/2 MS更适石斛组培苗生长;添加0.2 mg/L NAA对铁皮石斛组培苗的生长效果显著高于其他生长素;蔗糖质量浓度为25 g/L、无机盐浓度为1/2 MS时培养基渗透压环境更适合铁皮石斛组培苗生长;有机添加物LH(水解乳蛋白)和CH(水解酪蛋白)均对铁皮石斛组培苗生长有促进作用,其中添加0.1 g/L水解乳蛋白最适合铁皮石斛组培苗的生长,0.8 cm组培苗,培养60 d后植株平均株高2.26 cm、茎粗2.73 mm、生根量2.45条、分蘖量3.51株,植株生长旺盛,叶片大深绿色。本研究为建立高效铁皮石斛组培快繁奠定了基础。In this paper,the factors affecting the growth of Dendrobium officinale tissue culture seedlings were studied in order to optimize the rapid propagation system of Dendrobium officinale tissue culture.The results showed that low salt medium 1/2 MS was more suitable for the growth of Dendrobium tissue culture seedlings;the effect of adding 0.2 mg/L NAA on the growth of Dendrobium officinale tissue culture seedlings was significantly higher than other auxin;when sucrose concentration was 25 g/L and inorganic salt concentration was 1/2 MS,the osmotic pressure environment of culture medium was more suitable for the growth of Dendrobium officinale tissue culture seedlings;organic additives LH(hydrolyzed milk protein)and CH(hydrolyzed casein)could promote the growth of Dendrobium officinale tissue culture seedlings,among which 0.1 g/L hydrolyzed milk protein was the most suitable for the growth of Dendrobium officinale tissue culture seedlings,where 0.8 cm tissue culture seedlings,after 60 days of culture,reached the average plant height 2.26 cm,stem diameter 2.73 mm,rooting number 2.45,tillering amount 3.51,and the plant grew vigorously with the leaves large and dark green.This study lays a foundation for the establishment of efficient tissue culture and rapid propagation of Dendrobium officinale.

关 键 词：铁皮石斛 组培苗 生长 影响因子 

分 类 号：S685.99[农业科学—观赏园艺]