菌株ZYRH-19的筛选鉴定及其合成韦兰胶的特性研究  

Screening,identification of strain ZYRH-19 and characteristics of its product welan gum

作　　者：章阳 陆贺港 游鹏智 李晓雁 黄海东 Zhang Yang;Lu Hegang;You Pengzhi;Li Xiaoyan;Huang Haidong(Tianjin Agricultural University,College of Agronomy and Resources Environment,Tianjin 300392,China;Tianjin Agricultural University,College of Food Science and Bioengineering,Tianjin 300392,China)

机构地区：[1]天津农学院农学与资源环境学院,天津300392 [2]天津农学院食品科学与生物工程学院,天津300392

出　　处：《天津农学院学报》2024年第1期36-40,65,共6页Journal of Tianjin Agricultural University

基　　金：国家级大学生创新创业训练计划项目(202110061040);国家自然科学基金面上项目(31571790)。

摘　　要：从土壤中分离得到一株产胞外多糖的菌株ZYRH-19,从形态学、生理生化和16S rDNA基因序列的角度对其进行分类鉴定,并对其发酵合成的胞外多糖进行分析鉴别。结果表明:ZYRH-19为鞘氨醇单胞菌属菌株,与Sphingomonas sp.ATCC 31555同源性为99.93%,其合成的胞外多糖为韦兰胶,重均分子量为5.3×10^(6) u;常温下韦兰胶溶液具有良好的稀释剪切性,在6 r/min剪切率下黏度能达到1444.1 mPa·s,凝胶强度为22.6 g,在160℃下具有良好的抗温性能。研究表明,菌株ZYRH-19合成的韦兰胶性能更好,为韦兰胶的工业生产提供了新的菌种资源。In this study,a strain ZYRH-19 producing extracellular polysaccharide was isolated from soil.It was classified and identified from the perspectives of morphology,physiology,biochemistry,and 16S rDNA gene sequence,and the extracellular polysaccharide synthesized by strain ZYRH-19 was analyzed and identified.The results showed that ZYRH-19 was a strain of Sphingomonas sp.,and its homology with Sphingomonas sp.ATCC 31555 was 99.93%.The extracellular polysaccharide synthesized by strain ZYRH-19 was welan gum with weight average molecular weight of 5.3×10^(6) u.The welan gum solution synthesized by ZYRH-19 had good dilution shear property at room temperature.The viscosity of the product solution could reach 1444.1 mPa·s at the 6 r/min shear rate,and its gel strength was 22.6 g.It had good temperature resistance at 160℃.The results showed that the performance of welan gum synthesized by strain ZYRH-19 is very good,which provides a new strain resource for the industrial production of welan gum.

关 键 词：鞘氨醇单胞菌 分类鉴定 韦兰胶 多糖 

分 类 号：S154.39[农业科学—土壤学]