蓖麻粕对黑水虻幼虫生长性能的影响  

Effect of castor meal on growth performance of Hermetia illucens larvae

作　　者：俞作建 乔奕宁 姚嘉诚 周俊锋[3] 王存文[2] YU Zuo-jian;QIAO Yi-ning;YAO Jia-cheng;ZHOU Jun-feng;WANG Cun-wen

机构地区：[1]武汉工程大学环境生态与生物工程学院,湖北武汉430205 [2]武汉工程大学绿色化工过程教育部重点实验室,湖北武汉430205 [3]武汉工程大学资源与安全工程学院兴发矿业学院,湖北武汉430205

出　　处：《饲料研究》2024年第4期74-78,共5页Feed Research

基　　金：国家自然科学基金面上项目“黑水虻降解转化抗生素的过程及其作用机制(项目编号:42077357)”;武汉工程大学研究生创新基金项目(项目编号:CX2022558);云南省科技厅浮选磷尾矿资源化利用关键技术研发项目(项目编号:202103AA080007)。

摘　　要：试验旨在探究蓖麻粕代替豆粕作为饲粮对黑水虻幼虫生长发育及其肠道微生物的影响。将黑水虻幼虫分为3组,分别是豆粕组(SM组)、50%豆粕和50%蓖麻粕组(SCM组)以及蓖麻粕组(CM组),每组3个重复,测定幼虫存活数、体重和体长等,检测分析幼虫和虫沙粗蛋白和粗脂肪含量、幼虫肠道细菌群落等变化。试验期12 d。结果显示,CM组虫沙的粗蛋白和粗脂肪含量显著低于其他两组(P<0.05)。16S核糖体(RNA)基因测序显示,蓖麻粕对幼虫肠道细菌的主要类型、丰富度和多样性无明显影响(P>0.05)。CM组的黑水虻幼虫生长更快,粗蛋白和粗脂肪含量显著高于SM组(P<0.05),而死亡率无显著差异(P>0.05)。研究表明,蓖麻粕代替豆粕作为饲粮更有利于黑水虻幼虫的生长。The experiment aimed to investigate the impact of replacing soybean meal with castor meal as a feed on the growth and development,as well as the influence on intestinal microorganisms of Hermetiaillucenslarvae.The Hermetia illucenslarvae were divided into three groups:Soybean meal group(SM group),50% soybean meal and 50%castor meal group(SCM group),and castor meal group(CM group),with three replicates for each group.Parameters such as larval survival,body weight,and body length were measured,and changes in crude protein and crude fat content in larvae and larval frass,as well as the intestinal bacterial community of larvae,were analyzed.The experiment lasted for 12 days.The results showed that the crude protein and crude fat content in the larval frass of the CM group were significantly lower than those in the other two groups(P<0.05).16S rRNA gene sequencing revealed that castor meal had no significant impact on the major types,abundance,and diversity of intestinal bacteria in the larvae(P>0.05).The Hermetiaillucenslarvae in the CM group exhibited faster growth,with significantly higher crude protein and crude fat content compared to the SM group(P<0.05),while the mortality rate showed no significant difference(P>0.05).The study suggests that replacing soybean meal with castor meal as a feed is more beneficial for the growth of Hermetiaillucenslarvae.

关 键 词：黑水虻 蓖麻粕 存活率 肠道微生物 生长曲线 

分 类 号：S816[农业科学—饲料科学]