两种高黎贡山附生兰的种子表面灭菌及生活力测定研究  

Comparative Study on Surface Sterilization Methods and Viability Determination of Two Epiphytic Orchid Seeds from Gaoligong Mountains

作　　者：李村富 浦秀丽 李春芳[1] 蒋宏 何俊[1] LI Cunfu;PU Xiuli;LI Chunfang;JIANG Hong;HE Jun(Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming Yunnan 650201,P.R.China;Yunnan Academy of Forestry and Grassland,Kunming Yunnan 650201,P.R.China)

机构地区：[1]中国科学院昆明植物研究所,中国西南野生生物种质资源库,云南昆明650201 [2]云南省林业和草原科学院,云南昆明650201

出　　处：《西部林业科学》2024年第1期1-7,24,共8页Journal of West China Forestry Science

基　　金：科技部国家科技基础资源调查专项(2021FY100200);云南省重点研发计划(202103AC100003)。

摘　　要：为筛选出一种便捷、高效的测定附生兰种子生活力的通用方法,对高黎贡山2种附生兰的种子进行不同表面灭菌处理,通过测定和比较分析种子的TTC染色率与非共生萌发率。结果显示:2种附生兰种子对灭菌试剂的种类和浓度存在耐受性差异。金耳石斛种子的灭菌耐受性较好,属于耐受型种子,4种灭菌试剂处理一段时间后种子均能保持较好的TTC染色率与非共生萌发率。长瓣万代兰种子属于灭菌不耐受型种子,4种灭菌试剂处理一段时间后种子染色率均下降,但2%NaClO、1%NaDCC处理后能保持较好的非共生萌发率。结果表明:2种附生兰种子用1%NaDCC处理10~40 min的萌发率均没有显著性差异,1%NaDCC处理10~40 min可作为二者最佳灭菌方法。In order to identify a convenient and efficient surface sterilization method for epiphytic orchid seeds,two species of epiphytic orchid seeds from Gaoligong Mountains were subjected to different surface sterilization methods.By comparing the consistency between the TTC staining rate and asymbiotic germination rate of the seeds.The resultSshowed that the two species of epiphytic orchid seeds had different tolerances to sterilization agents in terms of type and concentration.The seeds of Dendrobium hookerianum exhibited good tolerance to sterilization,belonging to the tolerant type of seeds,as they maintained good TTC staining rate and asymbiotic germination rate after treatment with 4 sterilization agents for a certain period of time.The seeds of Vanda longitepala were classified as non-tolerant seeds to sterilization.The staining rate of the seeds decreased after treatment with 4 sterilization agents for a certain period of time,but they maintained a good asymbiotic germination rate after treatment with 2%NaClO and 1%NaDCC.Based on comprehensive evaluation,treating both species of epiphytic orchid seeds with 1%NaDCC for 10-40 min showed no significant difference in germination rate,making it the optimal sterilization method for both species.

关 键 词：兰科 附生兰 TTC染色率 非共生萌发率 灭菌耐受性 

分 类 号：S682.31[农业科学—观赏园艺]