转抗虫基因107杨对根际和内源细菌群落的影响  

Effect of Insect-resistance Transgenic 107 Poplar on Rhizosphere and Endogenous Bacterial Communities

作　　者：路文然 任亚超[1,2] 赵津 王进茂 郭甫鑫[1,2] 张军 LU Wenran;REN Yachao;ZHAO Jin;WANG Jinmao;GUO Fuxin;ZHANG Jun(Agricultural University of Hebei College,Baoding Hebei 071000,P.R.China;Key Laboratory of Forest Germplasm ResourceSand Forest Protection,Baoding Hebei 071000,P.R.China)

机构地区：[1]河北农业大学林学院,河北保定071000 [2]河北省林木种质资源与森林保护重点实验室,河北保定071000

出　　处：《西部林业科学》2024年第1期72-81,共10页Journal of West China Forestry Science

基　　金：农业生物育种重大项目(2022ZD0401502);国家林业和草原局科技发展中心项目(KJZXSA2021104);河北农业大学引进人才科研专项(YJ2021028)。

摘　　要：为研究转抗虫基因对根际和内源细菌群落的影响,以转抗虫基因107杨田间对比试验林为对象,采用高通量测序技术对转基因株系和对照株系根际土壤细菌、树干韧皮部和根系内生细菌群落结构组成及多样性进行对比分析。结果显示:(1)土壤样品中有效序列平均在30000以上,杨树组织有效序列平均在300000以上。(2)转基因株系细菌物种组成与对照株系的极其相似,酸杆菌门与变形菌门为转基因和非转基因株系根际土壤的优势菌门,占比分别为16.6%和21.9%;蓝藻菌门和变形菌门为转基因和非转基因株系韧皮部的优势菌门,所占的比率分别为54.8%和59.2%;变形菌门为转基因和非转基因株系根部的优势菌门,所占的比率分别为40.7%和58.8%。(3)转基因和非转基因株系在细菌群落的丰富度和多样性上均没有表现出明显的差异,但不同部位之间差异显著。结果表明:转基因107杨没有对根际土壤和内生细菌造成显著影响,明确了转抗虫基因107杨不同部位细菌群落特征,为转抗虫基因107杨的生物安全评价提供了有益的参考。In order to research transgenic poplar 107 effect on in rhizosphere soil and control soil,we used high-throughput sequencing technology to compare and analyze the structure and diversity of soil bacteria,phloem and root endophytic fine communities of transgenic poplar 107 transgenic in rhizosphere soil and control soil.s equencing results showed that the effective sequences in soil samples averaged more than 30000,and those in poplar tissues averaged more than 300000.The species composition of transgenic strains is extremely similar to that of the control strain.In the rhizosphere soil samples,Acidobacteria and Proteobacteria was the dominant phyla of transgenic and non-transgenic strains,accounting for 16.6%and 21.9%,respectively.In phloem samples,Cyanobacteria and Proteobacteria were the dominant phyla of transgenic and non-transgenic strains,accounting for 54.8%and 59.2%,respectively.In the root samples,Proteobacteria were the dominant phyla of transgenic and non-transgenic strains,accounting for 40.7%and 58.8%,respectively.There was no significant difference in the richness and diversity of bacterial community between transgenic and non-transgenic strains,but there was significant difference between different parts.Through diversity analysis,it was found that the communities of transgenic and control strains had high similarity,while the communities of samples from different parts had certain differences.The results of this study showed that transgenic poplar 107 had no significant effects on rhizosphere soil and endophytic bacteria,and clearly presented the characteristics of bacterial communities in different parts of transgenic poplar 107,which provided a reference for biosafety evaluation of transgenic poplar 107.A reliable and practical method has been proposed to effectively address insect resistance in the cultivation of new transgenic poplar varieties.

关 键 词：转抗虫基因107杨 高通量测序 细菌群落 丰富度 多样性 

分 类 号：S792.11[农业科学—林木遗传育种]