基于HS-GC-MS及网络药理学技术解析艾叶不同炮制方法减毒的物质基础及分子机制  

Study on Material Basis and Molecular Mechanism of Attenuating Toxicity of Artemisiae Argyi Folium by Different Processing Methods Based on HS-GC-MS and Network Pharmacology

作　　者：郎士跃 耿雪 化敏 李念虹 高奇 王变利[2,4] 周倩 LANG Shi-yue;GENG Xue;HUA Min;LI Nian-hong;GAO Qi;WANG Bian-li;ZHOU Qian(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Academy of Chinese Medicine,Jinan 250014,China;Shandong Insititute for Food and Drug Control,Jinan 250101,China;Modern Research and Development of Aromatherapy in Traditional Chinese Medicine Shandong Engineering Research Center,Jinan 250014,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东省中医药研究院,山东济南250014 [3]山东省食品药品检验研究院,山东济南250101 [4]传统中医芳疗的现代化研究与开发山东省工程研究中心,山东济南250014

出　　处：《中药材》2023年第7期1655-1663,共9页Journal of Chinese Medicinal Materials

基　　金：山东省中药材及饮片标准研究(2020-247,2020-248)。

摘　　要：目的:基于艾叶、清炒艾叶、醋炙艾叶中挥发性成分的差异性,探究艾叶炮制减毒的物质基础和分子机制。方法:采用顶空-气相色谱-质谱(HS-GC-MS)法鉴定艾叶及炮制品中的挥发性成分;运用多元统计方法分析生品与炮制品的差异成分,解析其可能的减毒物质基础。借助网络药理学技术挖掘差异成分与肝毒性相关的靶点和通路,探究艾叶炮制减毒的分子机制。结果:实验共鉴定出41个化学成分,清炒后含量降低的差异成分有17个,醋炙后含量降低的差异成分有16个。网络药理学结果表明艾叶经清炒、醋炙后分别有10、12个成分通过作用于133、50个交集基因,并调控多条信号通路达到降低肝毒性的目的。结论:该研究初步阐释了艾叶不同炮制方法对挥发性成分的影响及减轻肝毒性的潜在分子机制,可为阐明艾叶炮制减毒的科学内涵提供实验依据和数据支持。Objective:To explore the material basis and molecular mechanism of attenuating toxicity in the processing of Artemisiae Argyi Folium based on the difference of volatile components in Artemisiae Argyi Folium and its stir-fry,vinegar samples.Methods:Head-space-gas chromatography-mass spectrometry(HS-GC-MS)was used to identify the volatile components in Artemisiae Argyi Folium and its products.Multivariate statistical methods were used to analyze the different components of raw and processed products,and to analyze the possible basis of attenuated substances.With the help of network pharmacology,the targets and pathways related to hepatotoxicity of different components were explored to explore the molecular mechanism of processing attenuated toxicities of Artemisiae Argyi Folium.Results:A total of 41 chemical components were identified.The contents of 17 different components decreased after stir-frying and 16 different components decreased after roasting with vinegar.The results of network pharmacology showed that 10 and 12 components of Artemisiae Argyi Folium acted on 133 and 50 intersection genes and regulated multiple signaling pathways to reduce liver toxicity.Con-clusion:This study preliminarily explains the effects of different processing methods of Artemisiae Argyi Folium on volatile components and the potential molecular mechanism of alleviating liver toxicity,which can provide experimental basis and data support for clarifying the scientific connotation of processing attenuated toxicities of Artemisiae Argyi Folium.

关 键 词：艾叶 炮制减毒 HS-GC-MS 多元统计分析 肝毒性 网络药理学 

分 类 号：R283.1[医药卫生—中药学] R285[医药卫生—中医学]