腰果CBF基因家族的鉴定及响应冷胁迫的表达模式  

Identification of CBF Gene Family in Cashew and Its Expression Pat�tern in Response to Cold Stress

作　　者：崔恒久 曾教科 李雯[1,4] 黄海杰[5,6] 彭世清 郭冬 蒲金基[2,3] 周永凯 李辉亮[2,3] CUI Hengjiu;ZENG Jiaoke;LI Wen;HUANG Haijie;PENG Shiqing;GUO Dong;PU Jinji;ZHOU Yongkai;LI Huiliang(College of Horticulture,Hainan University,Haikou,Hainan 570228,China;Hainan Academy of Tropical Agricultural Re�sources,Haikou,Hainan 571101,China;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agri�cultural Sciences,Haikou,Hainan 571101,China;Key Laboratory for Quality Regulation of Tropical Horticultural Crops ofHainan Province,Haikou,Hainan 570228,China;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Ag�ricultural Sciences,Haikou,Hainan 571101,China;National Cultivar Improvement Center of Tropical Fruit Tree,Haikou,Hainan571101,China)

机构地区：[1]海南大学园艺学院,海南海口570228 [2]海南热带农业资源研究院,海南海口571101 [3]中国热带农业科学院热带生物技术研究所,海南海口571101 [4]海南大学海南省热带园艺作物品质调控重点实验室,海南海口570228 [5]中国热带农业科学院热带作物品种资源研究所,海南海口571101 [6]国家热带果树品种改良中心,海南海口571101

出　　处：《热带作物学报》2024年第3期473-480,共8页Chinese Journal of Tropical Crops

基　　金：海南省热带园艺作物品质调控重点实验室开放课题[No.HNZDSYS(YY)-02];海南省自然科学基金项目(No.322RC799);国家科技资源共享服务平台项目“国家热带植物种质资源库”。

摘　　要：腰果(Anacardium occidentalie Linn)属于典型的热带果树,主要在我国的云南省、海南省等地种植。腰果具有重要的经济价值、社会价值、生态价值,为农民增产创收,实现共同富裕和乡村振兴战略具有重要价值。腰果对于低温十分敏感,在8℃时就会受到严重伤害,生长停止。所以低温胁迫是制约腰果生长的重要非生物胁迫。CBF转录因子是植物在应对低温、干旱、盐等非生物胁迫下中发挥重要作用的转录因子。然而CBF基因家族在腰果中的作用尚未阐明,该家族在低温胁迫下的表达模式尚不清楚。本研究对腰果AoCBF基因家族的基本特性进行探究,通过生物信息学方法,在腰果转录组中鉴定并获得6个AoCBF基因家族成员,分别对其系统发育、理化性质、蛋白结构、低温诱导下表达模式和启动子作用元件进行分析。理化性质分析结果表明:6个AoCBF蛋白长度为189~334 aa,蛋白分子量为21.31~37.89 kDa,等电点均小于7,表明该蛋白呈酸性,且均包含AP2/ERF结构域。基序分析表明:AoCBF基因存在5个基序,不同AoCBF基因Motif的缺失暗示着相关功能的缺失性。系统发育树分析表明:6个AoCBFs可分为A、B两个亚组,其中A组包含1个基因,B组仅包含5个基因。启动子顺势作用元件分析得出,AoCBF可能参与激素响应及非生物胁迫响应过程。荧光定量表达分析显示,AoCBFs对低温冷胁迫均有不同程度的响应,表明AoCBF在响应低温胁迫生物学过程中发挥着一定的作用。本研究分析了AoCBF基因家族的基本特性,为后续AoCBF的深入研究和应用积累基础数据。Anacardium occidentalie Linn is a typical tropical fruit tree,which is mainly planted in Yunnan and Hainan provinces of China.Cashew cultivation has important economic,social and ecological values.It is of great value to realize realize the strategy of rural revitalization in China,increase farmers'production and income,and achieve common prosperity.Cashews are very sensitive to low temperatures,and they will be severely damaged at 8℃and the growth will stop.Therefore,lowtemperature stress is an important abiotic stress that restricts cashew growth.CBF transcription factors play important roles in plant response to abiotic stresses such as low temperature,drought and salt.However,the role of CBF gene family in cashews has not been elucidated,and the expression pattern of this family in response to low temperature stress is unknown.In this study,in order to explore the basic characteristics of the CBF gene family in cashew nuts,six AoCBF gene family transcription factors were identified and obtained from the cashew transcriptome by bioinformatics methods,and the physical and chemical properties,protein structure,phylogeny,promoter action elements and expression patterns under low temperature induction were analyzed.The length of the six AoCBF proteins ranged from 189 to 334 aa,and the molecular weight of the proteins ranged from 21.31 to 37.89 kDa.The isoelectric points of the six AoCBF proteins were less than 7,indicating that the proteins were acidic.The proteins all contained an AP2/ERF domain.Motif analysis showed that there were five motifs in AoCBF genes,and the absence of different AoCBF gene motifs implied the absence of related functions.Phylogenetic tree analysis showed that the six AoCBF genes could be divided into two subgroups,A and B,in which group A contained one gene and group B only contained five genes.The analysis of homeotropic elements of promoter showed that AoCBF gene might be involved in the response to hormone and abiotic stress.Fluorescence quantitative expression analysis showed that a

关 键 词：腰果 低温响应 CBF转录因子 基因家族 QRT-PCR 

分 类 号：S667.9[农业科学—果树学]