槟榔籽提取物对血管紧张素转化酶(ACE)活性的抑制作用研究  

Inhibitory Effects of Areca Nut Extract on the Activity of Angiotensin Converting Enzyme

作　　者：于鑫 唐敏敏[2,3] 罗家欣 宋菲 朱婷婷[4] 陈华 赵松林 YU Xin;TANG Minmin;LUO Jiaxin;SONG Fei;ZHU Tingting;CHEN Hua;ZHAO Songlin(College of Food Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China;Coconut ResearchInstitute,Chinese Academy of Tropical Agricultural Sciences,Wenchang,Hainan 571339,China;Hainan Betel Nut EngineeringTechnology Research Center,Wenchang,Hainan 571339,China;College of Food Science and Engineering,Hainan TropicalOcean University,Sanya,Hainan 572022,China)

机构地区：[1]华中农业大学食品科学与技术学院,湖北武汉430070 [2]中国热带农业科学院椰子研究所,海南文昌571339 [3]海南省槟榔工程技术研究中心,海南文昌571339 [4]海南热带海洋学院食品科学与工程学院,海南三亚572022

出　　处：《热带作物学报》2024年第3期592-601,共10页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(No.322RC791);海南省重点研发计划项目(No.ZDYF2022XDNY205)。

摘　　要：以嫩果期槟榔籽为研究对象,采用75%乙醇浸提制得槟榔籽提取物(areca nut extract,ANE),再分别用石油醚、乙酸乙酯和正丁醇依次对总提取物进行萃取,依次得到嫩果期槟榔籽75%乙醇提取物的石油醚部位(PE-ANE)、乙酸乙酯部位(EAC-ANE)、正丁醇部位(BU-ANE)、水部位(W-ANE)的萃取部分。通过体外抑制血管紧张素转化酶(ACE)活性抑制试验测定槟榔提取物各极性部位对高血压的抑制潜力;通过酶动力学实验分析ACE抑制活性最好的极性部位对ACE活性的抑制作用类型;测定不同极性部位蛋白质、总糖及多酚的总含量,并分析其与ACE半抑制浓度(IC50)之间的相关性;最后对4种不同极性部位萃取物中的多酚组分进行初步定量。结果表明:嫩果期槟榔籽提取物各极性部位均具有一定的ACE抑制活性,其中EAC-ANE的ACE抑制活性最好。酶动力学实验表明,EAC-ANE对ACE活性的抑制作用类型为竞争与非竞争的混合型抑制。相关性分析表明,各极性部位ACE的IC50值与多酚、总糖及蛋白质含量均呈显著负相关,且与多酚含量之间具有极强相关性(r=‒0.912)。在4个极性部位中定量了儿茶素、L-表儿茶素、原花青素B2、原儿茶酸、槲皮素等多种多酚组分,且EAC-ANE中的相关多酚组分含量最高。综上所述,槟榔提取物具有一定的降血压潜力,且乙酸乙酯萃取部位最好,多酚含量与各萃取部位的ACE抑制活性具有极强的相关性,可初步推断多酚是槟榔提取物中抑制ACE活性的重要物质。In this study,75%ethanol solution was used to prepare the total extracts of arecanut nut(ANE).Then different fractions of the extracts were prepared by fractionating with petroleum ether(PE-ANE),ethyl acetate(EAC-ANE),n-butanol(BU-ANE)and water(W-ANE)in turn.The inhibition of hypertension from ANE was determined by angiotensin converting enzyme(ACE)activity inhibition assay in vitro.The inhibitory was evaluated with the fraction displayingthe best ACE inhibitory activity by the enzyme kinetic method.In addition,the contents of total polyphenols,total sugar and total protein in each fraction of ANE were determined.The correlation between the contents of the compositions and ACE inhibitory activities was analyzed.Finally,phenoliccomponents in the four fractions were ana-lyzed.The results showed that all the fractions of ANE at different polar parts exhibited ACE inhibitory activity,and EAC-ANE displayed the best.Enzyme kinetics tests showed that the inhibitory effect of EAC-ANE from tender areca on ACE was a mixed type of competitive inhibition and non-competitive inhibition.The correlation analysis showed that the IC50 value of the ACE inhibitory activity of each polar part of ANE was significantly negatively correlated with the content of polyphenols and total sugar.And the absolute value of the correlation coefficient between the IC50 value of ACE inhibitory activity and the polyphenol content reached a very strong correlation(r=‒0.912).And a variety of polyphenol components such as catechin,L-epicatechin,procyanidin B2,protocatechuic acid,and quercetin were identified in the four different polar parts,and the content of polyphenol components in EAC-ANE was the highest.In conclusion,ANE has a certain blood pressure lowering potential,and the ethyl acetate extraction site is the best,and the polyphenol content has a strong correlation with the ACE inhibitory activity of each extract.It can be preliminarily inferred that polyphenols may be the main substances that inhibit the activity of ACE in ANE.

关 键 词：槟榔提取物 血管紧张素转化酶 抑制作用 酶动力学 

分 类 号：R285[医药卫生—中药学]