不同基质配比下黄瓜根际细菌群落结构及多样性分析  

Analysis of Cucumber Rhizosphere Bacterial Community Structure and Diversity under Different Matrix Ratios

作　　者：陈宏昊 唐小付[1] 聂圣贤 林佳佳 魏西 刘婷 任昊奎 CHEN Honghao;TANG Xiaofu;NIE Shengxian;LIN Jiajia;WEI Xi;LIU Ting;REN Haokui(College of Agronomy,Guangxi University,Nanning,Guangxi 530004,China)

机构地区：[1]广西大学农学院,广西南宁530004

出　　处：《热带作物学报》2024年第3期641-652,共12页Chinese Journal of Tropical Crops

基　　金：广西重点研发计划项目(桂科AB1850031,桂科AB19245016,桂科AB23026097)。

摘　　要：分析菇渣、锯末不同比例配比下黄瓜植株根际细菌群落结构特征发现,菇渣和锯末复配基质可替代常规商品基质泥炭(CK)栽培黄瓜,为新型基质栽培模式的应用提供依据。与CK相比,用菇渣、锯末按照3种不同比例复配组成的基质栽培黄瓜,其产量、根际细菌多样性和丰富度指数间均显著提高,同时还影响根际细菌的代谢功能,其中菇渣与锯末比例为1∶1时(T2)表现最佳。在门分类水平上,菇渣、锯末复配基质栽培条件下黄瓜植株根际中变形菌门(Proteobacteria)细菌丰度占比均低于CK,但拟杆菌门(Bacteroidota)细菌丰度占比均高于CK,且拟杆菌门(Bacteroidota)是菇渣、锯末复配基质栽培共有的优势细菌门类。在属分类水平上,unclassified_c__Gammaproteobacteria、利姆诺杆菌属(Limnobacter)和朱氏杆菌属(Chujaibacter)是CK条件下黄瓜植株根系特有的优势细菌属;慢生根瘤菌属(Bradyrhizobium)、短波单胞菌属(Brevundimonas)、Dokdonella属是菇渣与锯末比例为1∶3(T1)处理下特有的优势细菌属;菇渣与锯末比例分别为1∶1(T2)和3∶1(T3)条件下黄瓜植株根际未发现特有的优势细菌属。通过物种Venn分析,在种分类水平上,菇渣与锯末复配比例在1∶3(T1)、1∶1(T2)和3∶1(T3)处理下均增加了黄瓜植株根际细菌种分类水平数量,提升了根际细菌丰富度和多样性。The structural characteristics of the rhizosphere bacterial communities of cucumber plants under different proportions of mushroom residue and sawdust were analyzed,and it was found that mushroom residue and sawdust compound substrate could replace conventional commercial matrix peat(CK)cultivation cucumber,which providing a basis for the application of a new substrate cultivation mode.Compared with CK,cucumber cultivated with substrate composed of mushroom residue and sawdust in three different proportions significantly increased its yield,rhizosphere bacterial diversity and richness index,and also affected the metabolic function of rhizosphere bacteria,among which the ratio of mushroom residue to sawdust was 1∶1(T2).At the classification level,the proportion of bacterial abundance in Proteobacteria of cucumber plants under the cultivation of mushroom residue and sawdust compound substrate was lower than that of CK,but the proportion of bacterial abundance of Bacteroidota was higher than that of CK,and Bacteroidota was the dominant bacterial phylum shared by mushroom residue and sawdust compound substrate cultivation.At the taxonomic level,unclassified_c__Gammaproteobacteria,Limnobacter and Chujaibacter were the dominant bacterial genera unique to the root system of cucumber plants under CK conditions.Bradyrhizobium,Brevundimonas,and Dokdonella were the dominant bacterial genera with a 1∶3(T1)ratio of mushroom residue to sawdust.Under the con ditions of 1∶1(T2)and 3∶1(T3)ratios of mushroom residue to sawdust,respectively,no dominant bacterial genus was found in the rhizosphere of cucumber plants.Through species Venn analysis,the ratio of mushroom residue to sawdust at the species classification level increased the number of rhizobacteria species in cucumber plants under the treatment of 1∶3(T1),1∶1(T2)and 3∶1(T3),and the richness and diversity of rhizosphere bacteria were improved.

关 键 词：黄瓜 高通量测序 细菌群落结构 功能预测 

分 类 号：S642.2[农业科学—蔬菜学]