不同光照时长处理对柚果皮有色层香豆素代谢的影响  

Effect of Treatments at Different Light Time on Coumarin Metabolism in Colored Layer of Pomelo Pericarp

作　　者：雷旭 黄迪捷 周青青 邓志军 邓仕明 牟蛟琳 LEI Xu;HUANG Dijie;ZHOU Qingqing;DENG Zhijun;DENG Shiming;MOU Jiaolin(Hubei Key Laboratory of Biologic Resources Protection and Utilization,Hubei Minzu University,Enshi,Hubei 445000,China;College of Forestry and Horticulture,Hubei Minzu University,Enshi,Hubei 445000,China;Research Center for Germplasm Engineering of Characteristic Plant Resources in Enshi Prefecture,Hubei Minzu University,Enshi,Hubei 445000,China)

机构地区：[1]湖北民族大学生物资源保护与利用湖北省重点实验室,湖北恩施445000 [2]湖北民族大学林学园艺学院,湖北恩施445000 [3]湖北民族大学恩施州特色植物资源种质工程技术研究中心,湖北恩施445000

出　　处：《天津农业科学》2024年第3期1-9,共9页Tianjin Agricultural Sciences

基　　金：恩施州科技厅青年人才项目(XYJ202200089)。

摘　　要：柚是我国南方地区重要的水果,表皮富含大量的香豆素,极易受到外界环境的影响,但其光响应效果尚不明确。为探究香豆素在柚表皮中的光响应机制,通过设置12、24、36、48 h共4个不同光照时长处理来探究光照对柚果皮有色层香豆素代谢的影响。本研究以贡水白柚和矮晚柚为试材,采用靶向代谢组学的检测方法共检测到21种香豆素,随后对贡水白柚、矮晚柚对照组和处理组果皮有色层中香豆素的代谢差异进行比较分析。结果表明:在贡水白柚和矮晚柚果皮有色层共同积累的21种香豆素中,相对含量较高且存在显著差异的香豆素为佛手柑素、橙皮油内酯、蛇床子素。不同光照时长处理对贡水白柚果皮有色层中佛手柑素、橙皮油内酯、蛇床子素、总香豆素的积累有显著影响,对矮晚柚果皮有色层中王草酚、佛手柑素、橙皮油内酯、蛇床子素、总香豆素的积累有显著影响。通过PCA分析显示,经过不同光照时长处理的贡水白柚和矮晚柚果皮有色层中总香豆素的相对含量被明显区分开。综上,光照处理会改变贡水白柚和矮晚柚果皮有色层中香豆素的积累特征,并且矮晚油相较贡水白柚大量积累王草酚和二氢欧山芹素。以上研究结果为揭示光照对柑橘香豆素代谢的调节机制奠定了基础,为提升柑橘药用品质提供了全新的思路。Pomelo,a significant fruit in southern regions,contains coumarin-rich pericarp highly influenced by the external environment.However,the impact of light on coumarin metabolism remains unclear.This study conducted four light time treatments(12 h,24 h,36 h,and 48 h)to investigate light's effect on coumarin metabolism in the colored layer of pomelo pericarp.Gongshui white pomelo and dwarf-late pomelo were utilized as test materials,with 21 coumarins detected through targeted metabolomics.Following this,pericarps were colored in control and treatment groups for comparative analysis of coumarin metabolic differences.The results indicated that bergamottin,aurapten and osthol were among the coumarins with high content and significant differences in the colored layer of Gongshui white pomelo and dwarf-late pomelo pericarps.Different light time significantly influenced the accumulation of bergamottin,aurapten,osthol and total coumarin in Gongshui white pericarp's colored layer.Additionally,osthenol,bergamottin,aurapten,osthol and total coumarin accumulation were notably affected in dwarf-late pomelo pericarp's colored layer.PCA analysis revealed distinct relative content of total coumarin in the colored layer of Gongshui white pomelo and dwarf-late pomelo treated with different light times.Overall,light treatment altered the coumarin accumulation characteristics in the colored layer of Gongshui white pomelo and dwarf-late pomelo,with dwarf-late pomelo showing higher osthenol and columbianetin accumulation compared to Gongshui white pomelo.These findings provide a basis for understanding the regulatory mechanism of light on citrus coumarin metabolism,it provides a new idea for improving the medicinal quality of citrus.

关 键 词：柚 果皮 香豆素 光照时间 靶向代谢组学 

分 类 号：S666.3[农业科学—果树学]