Three rare anti-inflammatory sesquiterpene lactones from Magnolia grandiflora  

作　　者：XU Shuangyu ZHANG Feng TAO Linlan JIANG Yangming HUANG Tao LI Yanan HU Zhanxing YANG Jue HAO Xiaojiang YUAN Chunmao 

机构地区：[1]State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550014,China [2]School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550025,China [3]Natural Products Research Center of Guizhou Province,Guiyang 550014,China [4]State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China

出　　处：《Chinese Journal of Natural Medicines》2024年第3期265-272,共8页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.82060631 and 32270413);the Science and Technology Department of Guizhou Province[Nos.QKH JC(2020)1Z076 and QKH JC(2020)1Y400],the Cultivation Project of National Natural Science Foundation of Guizhou Medical University(No.2023-106);the Outstanding Young Scientific and Technological Talents in Guizhou Province[No.QKH PTRC(2021)5633],High-level Innovative Talents in Guizhou Province(Thousand Levels of Talent for YUAN Chunmao in 2018);“Light of the West”Talent Cultivation Program of Chinese Academy of Sciences for YUAN Chunmao[RZ(2020)82],Guizhou Science and Technology Innovation Talent Team(No.QKHPTRCCXTD[2022]007);the Natural Science Foundation of Guizhou Province[No.QKHZYD(2022)4015].

摘　　要：Four new sesquiterpene lactones(SLs)(1–4),along with a biosynthetically related SL(5),have been isolated from the leaves of Magnolia grandiflora.Magrandate A(1)is notable as the first C18 homogemarane type SL,featuring a unique 1,7-dioxaspiro[4.4]nonan-6-one core.Compounds 2 and 3,representing the first instances of chlorine-substituted gemarane-type SL analogs in natural products,were also identified.The structures of these isolates were elucidated through a combination of spectroscopic data analysis,electronic circular dichroism calculations,and X-ray single-crystal diffraction analysis.All isolates demonstrated anti-inflammatory activity in lipopolysaccharide-stimulated RAW264.7 cells.Notably,3–5 showed a significant inhibitory effect on nitric oxide production,with IC50 values ranging from 0.79 to 4.73μmol·L^(−1).Additionally,4 and 5 exhibited moderate cytotoxic activities against three cancer cell lines,with IC50 values between 3.09 and 11.23μmol·L^(−1).

关 键 词：Magnolia grandiflora Sesquiterpene lactones Isolation and identification Anti-inflammatory activity 

分 类 号：R284.1[医药卫生—中药学]