芥菜bZIP基因家族的鉴定和表达分析  

Identification and Expression Analysis of bZIP Gene Family in Brassica juncea

作　　者：代春艳 张笑晗 王晓丽 弓琼 苏醒 成耀华 张明政 于澄宇[1] Dai Chunyan;Zhang Xiaohan;Wang Xiaoli;Gong Qiong;Su Xing;Cheng Yaohua;Zhang Mingzheng;Yu Chengyu(College of Agronomy,Northwest A&F University,Yangling,712100)

机构地区：[1]西北农林科技大学农学院,杨凌712100

出　　处：《分子植物育种》2024年第7期2104-2118,共15页Molecular Plant Breeding

基　　金：陕西省重点研发计划项目(2021NY-074)资助。

摘　　要：碱性亮氨酸拉链(bZIP)是一类广泛存在真核生物中的转录因子家族,参与多种植物生长发育及生物和非生物胁迫的响应过程,对下游许多基因的表达具有强烈调控作用。为了解芥菜bZIP基因家族的基因结构,成员之间的相互关系以及在非生物胁迫下的反应机制。本研究利用生物信息学方法,从Brassica Database (BRAD)数据库下载的芥菜基因组中鉴定到81个BjubZIP基因,其中34.57%的基因缺乏内含子,59.26%的BjubZIP等电点小于7,表明芥菜中有超过一半的bZIP蛋白为酸性蛋白,亚细胞定位预测所有的基因均位于细胞核。将81个BjubZIP进一步分为与拟南芥基因相对应的6个亚群,同一亚群的motif数量和基因结构高度相似。利用公共数据库中的芥菜转录组数据,对BjubZIP基因家族在干旱和高温胁迫下的表达分析,发现A亚群基因中的BjubZIP02、BjubZIP21、BjubZIP28、BjubZIP38、BjubZIP50、Bjub ZIP62、BjubZIP59在高温和干旱胁迫下均处于上调状态,推测芥菜中的这7个基因可能与拟南芥A亚群基因的功能相似,参与了在非生物胁迫下相应的生理过程。本研究结果可为今后芥菜bZIP家族成员的基因克隆和在胁迫反应中的功能研究提供参考。Basic leucinezipper(bZIP)is a family of transcription factors widely existing in eukaryotes.It isinvolved in the growth and development of many plants and the response process to biotic and abiotic stresses,and has a strong regulatory role in the expression of many downstream genes.The aim of this study is to understand the gene structure of bZIP gene family in mustard,the relationship among members and the response pattern under abiotic stress.A total of 81 BjubZIPs genes were identified from Brassica Database(BRAD)by bioinformatics method.It was found that 34.57%of them lack introns,and 59.26%of them have isoelectric point less than 7,which indicates that more than half of bZIP proteins are acidic proteins.Subcellular localization predicts that all genes are located in the nucleus.The 81 BjubZIPs were further divided into 6 subgroups corresponding to Arabidopsis genes.The motif number and gene structure of the same subgroup were highly similar.Using the transcriptome data of Brassica juncea in public database,the expression of BjubZIP gene family under drought and high temperature stress was analyzed.It was found that BjubZIP02,BjubZIP21,BjubZIP28,BjubZIP38,BjubZIP50,BjubZIP62 and BjubZIP59 in subgroup A were up-regulated under high temperature and drought stress.It is speculated that these seven genes may have similar functions to those of a subgroup genes in Arabidopsis thaliana and participate in the correspon-ding physiological processes under abiotic stress.The results of this study can provide a reference for the gene cloning of bZIP family members in mustard and the function research in stress response.

关 键 词：芥菜(Brassica juncea L.) BZIP 基因家族 表达分析 

分 类 号：S637[农业科学—蔬菜学]