乙烯利处理文心兰花瓣转录组分析及花衰老相关基因挖掘  

Transcriptome Analysis of Ethephon-treated Oncidium Petals and Discovery of Flower Senescence-related Genes

作　　者：裴小华 赵龙 田阳阳 王帅[1] 薛唯佳 曲飞鸿 翟珂 吴双 李梦桃 张恒[1] 潘英文[2] 夏志强 刘进平[1] Pei Xiaohua;Zhao Long;Tian Yangyang;Wang Shuai;Xue Weijia;Qu Feihong;Zhai Ke;Wu Shuang;Li Mengtao;Zhang Heng;Pan Yingwen;Xia Zhiqiang;Liu Jinping(Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources,College of Tropical Crops,Hainan University,Haikou,570228;Post-Entry Quarantine Station for Tropical Plant,Hainan Entry-Exit Inspection and Quarantine Bureau,Haikou,570311)

机构地区：[1]海南大学热带作物学院,海南省热带生物资源可持续利用重点实验室,海口570228 [2]海南出入境检验检疫局,热带植物隔离检疫中心,海口570311

出　　处：《分子植物育种》2024年第7期2127-2136,共10页Molecular Plant Breeding

基　　金：海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC147);海南省重点研发计划项目(ZDYF2020053);五指山市科技和工业信息化局项目(WZSKTPXM2021005);国家自然科学基金项目(31560573)共同资助。

摘　　要：为挖掘花衰老相关基因,揭示乙烯调控花瓣衰老的分子机制,利用乙烯利处理文心兰南茜种(Oncidium gower ramsey)盛开前期的切花花瓣,并进行RNA测序和转录组分析。测序结果共得到234 619个unigene。利用NR、GO、COG/KOG、KEGG和Swissprot数据库分别进行基因功能注释,分别注释到31 027 (77.22%)、11 126 (27.69%)、10 152 (25.27%)、5 523 (13.75%)和18 830 (46.86%)条unigenes。检测到差异表达基因有7 319条。GO功能富集分析共有4 622条差异表达基因注释到生物过程、细胞组分和分子功能3个功能群组;KEGG功能富集分析将差异表达基因主要参与15个代谢通路,其中植物激素信号转导富集的基因最多。鉴定出一批可能在乙烯调控文心兰花瓣衰老中发挥作用的植物激素信号转导关键成分基因和转录因子基因。本研究所获得的乙烯调控文心兰花瓣衰老转录组数据,有助于了解花衰老相关基因功能和阐明花衰老分子机制。In order to characterize flower senescence-related genes and clarify the molecular mechanism of ethylene-regulated petal senescence,RNA sequencing and transcriptome analysis were performed with the petals of ethephon-treated Oncidium gower ramsey cut flowers prior to full opening.A total of 234619 unigenes were obtainedbysequencing.Using NR,GO,COG/KOG,KEGG and Swissprot databases,we annotated 31027(77.22%),11126(27.69%),10152(25.27%),5523(13.75%)and 18830(46.86%)genes.Of all,7319 differentially expressed genes(DEGs)were detected.In GO function enrichment analysis,4622 DEGs were annotated into three functional groups:biological process,cell component and molecular function.The KEGG function enrichment analysis showed that the DEGs are mainly involved in 15 metabolic pathways,among which the DEGs were enriched most in plant hormone signal transduction pathways.A number of key component genes of plant hormone signal transduction and transcription factor genes that may play a role in Oncidium petal senescence of ethylene regulation were identified.The transcriptome data of Oncidium petal senescence of ethylene regulation obtained in this study is helpful to understand the function of flower senescence-related genes and reveal the molecular mechanism of flower senescence.

关 键 词：文心兰 乙烯 花瓣衰老 RNA测序 转录组 

分 类 号：S682.31[农业科学—观赏园艺]