光谱分析结合纸层析法对3个色系月季花色素组分鉴定  

Spectrometry Combined Paper Chromatography Identify the Components of Three Chromatic Chinese Rose Anthocyanidins

作　　者：李元鹏 张英杰[1] 朱志奇 张京伟[1] 郭文姣[1] 孙纪霞[1] Li Yuanpeng;Zhang Yingjie;Zhu Zhiqi;Zhang Jingwei;Guo Wenjiao;Sun Jixia(Yantai Agricultural Science and Technology Institute,Yantai,265500;College of Life Science,Yantai University,Yantai,264005;Laizhou Hongshun Mei Flower Planting Technology,Co.,Ltd.,Laizhou,261400)

机构地区：[1]山东省烟台市农业科学研究院,烟台265500 [2]烟台大学生命科学学院,烟台264005 [3]莱州市宏顺梅花种植科技有限公司,莱州261400

出　　处：《分子植物育种》2024年第7期2241-2247,共7页Molecular Plant Breeding

基　　金：山东省农业科技资金(林业科技创新)项目;烟台市科技计划项目(2018NCGY056,2019MSGY112)共同资助。

摘　　要：为鉴定不同色系月季花色素种类,结合利用显色反应、纸层析法和光谱法,对红、黄、蓝紫色系的3个品种的月季花瓣中花色素的种类进行了初步分析。本研究表明,‘朝圣者’花瓣花色素主要是黄酮类物质,可能是查尔酮、橙酮、二氢黄酮、黄酮醇或黄酮醇化合物。‘梅朗口红’花瓣内花色素包括矢车菊-3,5-葡萄糖苷,及黄酮类化合物和查尔酮、二氢黄酮。‘微蓝’花色素主要是芍药-3-芸香糖苷,同时还可能含有查尔酮和二氢黄酮。且通过对花瓣盛花期p H值的测定,进一步推测‘微蓝’显蓝紫色的原因,可能是芍药素苷损失质子而形成了显蓝的醌型。In order to identify the anthocyanidins of different colors,the anthocyanidins in petals of three varieties of Chinese rose from red,yellow and blue-purple were analyzed by using the methods of chromogenic reaction,paper chromatography and spectroscopy.The results showed that the anthocyanidins of'The Pilgrim'petals were mainly flavonoids,which might be chalcone,oleanone,dihydroflavone,flavonol or flavonol compounds.Antho-cyanidins in the petals of'Rouge Meilland'included cornflower centaur-3,5-glucoside,flavonoids compounds,chalcone and dihydroflavones.The'Kinda Blue'anthocyanidins were might paeoniflorin-3-rue glycoside,but may also contain chalcone and dihydroflavones.Through the measurement of the pH value of the petals at the full blos-som stage,it was further speculated that the reason of the'Kinda Blue'appears and bluish purple might be that paeoniflorin lost protons and formed bluish quinone type.

关 键 词：月季 显色反应 纸层析法 光谱法 花色素 

分 类 号：S685.12[农业科学—观赏园艺]