干旱胁迫下沙棘CMO基因的时空表达模式与蛋白结构预测  

Spatiotemporal Expression Patterns and Protein Structure Prediction of Seabuckthorn CMO Gene Under Drought Stress

作　　者：刘青青 马玉花[1] 董佳伟 冶贵生[1] 张丹 杨开宇 LIU Qing-qing;MA Yu-hua;DONG Jia-wei;YE Gui-sheng;ZHANG Dan;YANG Kai-yu(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,Qinghai,China)

机构地区：[1]青海大学农牧学院,青海西宁810016

出　　处：《西北林学院学报》2024年第2期21-27,共7页Journal of Northwest Forestry University

基　　金：国家自然科学基金(31660071);青海省科技厅项目(2017-ZJ-734)。

摘　　要：CMO基因是沙棘体内甜菜碱合成过程中的关键性基因,对植物体抗旱起着重要作用。以青海野生中国沙棘的根、茎、叶为试验材料,对沙棘CMO基因及其全长序列进行克隆,并进行生物信息学分析,在此基础上对不同程度干旱胁迫下沙棘不同组织部位CMO基因的时空表达模式进行研究。结果表明,沙棘CMO基因全长1566 bp,ORF长1364 bp,编码454个氨基酸,分子量为51.41 ku,等电点为6.78;CMO蛋白亚细胞定位于叶绿体,无信号肽,蛋白二级结构主要为无规则卷曲;CMO蛋白具有多个多种类型的功能位点,为蛋白功能的实现提供了保障;此外CMO蛋白的亲水性较强,无跨膜螺旋区;CMO蛋白三维结构由α螺旋、β折叠和无规则卷曲互相盘绕而成;时空表达模式研究表明沙棘CMO基因的表达明显受到干旱胁迫的影响,CMO基因的表达量随胁迫时间的延长而整体呈逐步升高的趋势,直至胁迫末期或复水后才有所降低;CMO基因在沙棘不同组织部位中的表达有一定的差异,表达量大小为根>叶>茎。通过对沙棘CMO基因的研究分析,以期为提高沙棘抗旱性及植物CMO基因的深入研究提供参考意义。CMO gene is a key gene for the synthesis of betaine in Hippophae rhamnoides.It plays an important role in drought resistance of plants.Taken the roots,stems and leaves of wild H.rhamnoides subsp.sinensis Rousi occurring in Qinghai as the experimental materials,the CMO gene and its full-length sequence were cloned and bioinformatics was analyzed,the spatiotemporal expression patterns of CMO genes in different tissue parts of sea buckthorn plant under different degrees of drought stress were studied.The results were reported as follows.The total length of CMO gene is 1566 bp,the ORF length is 1364 bp,encoding 454 amino acids,the molecular weight is 51.41 ku,the isoelectric point is 6.78;CMO protein subcells are localized in chloroplasts,no signal peptides,and the protein secondary structure is mainly irregular curling;CMO protein has a number of various types of functional sites,which provide a guarantee for the realization of protein function;in addition,CMO protein has strong hydrophilicity and no transmembrane helix region;the three-dimensional structure of CMO protein consists ofαhelix,βsheet,and random coil.The spatiotemporal expression pattern study showed that the expression of CMO gene was obviously affected by drought stress,and the expression of CMO gene showed a gradual increase with the extension of stress time,and did not decrease until the end of stress or after rehydration.The expression of CMO gene in different tissue parts was different,and the expression size was in the order of root>leaf>stem.This study analyzes the CMO gene of sea buckthorn in order to provide reference significance for improving the drought resistance of H.rhamnoides and the in-depth study of plant CMO gene.

关 键 词：沙棘 干旱胁迫 CMO基因 蛋白质结构 

分 类 号：S793.6[农业科学—林木遗传育种]