甘蓝型油菜RIL群体苗期根系耐铝性鉴定及评价方法研究  

Study on Identification and Evaluation Method of Aluminum Tolerance of Roots at Seedling Stage in RIL Population of Brassica napus L.

作　　者：李晨阳 史红松 徐璐 邢明礼 武晓如 白岩松 牛梦缘 高俊奇 李加纳[1] 周清元[1] 崔翠[1] LI Chenyang;SHI Hongsong;XU Lu;XING Mingli;WU Xiaoru;BAI Yansong;NIU Mengyuan;GAO Junqi;LI Jiana;ZHOU Qingyuan;CUI Cui(College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学农学与生物科技学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2024年第4期24-36,共13页Journal of Southwest University(Natural Science Edition)

基　　金：财政部和农业农村部国家现代农业产业技术体系项目(CARS-12);重庆市技术创新与应用发展项目(cstc2019jscx-msxmX0383).

摘　　要：为减少铵肥的大量施用以及酸雨频降引发的耕地酸化,从而影响作物的生长,利用综合评价方法通过幼苗期根系表型筛选耐铝种质.以甘蓝型油菜重组自交系(RIL)亲本10D130和中双11为材料筛选苗期适宜的铝胁迫浓度,使用该浓度处理RIL内138个品种(系)幼苗,对其根长、根表面积、根平均直径、根体积、根交叉数、根尖数和根干质量7个根系指标的铝毒单项耐性系数进行变异系数分析、相关性分析、主成分分析、系统聚类分析和逐步回归分析,并采用隶属函数法综合评价不同油菜品种(系)根系的耐铝特性.结果表明:138份油菜品种(系)间各项根系指标差异有统计学意义(p<0.05);筛选出甘蓝型油菜幼苗期耐铝毒品种(系)27034,27036和27007等.根据灰色关联度及逐步回归分析结果,认为根据根长、根表面积、根体积、根交叉数等7个关键指标,可以初步判断甘蓝型油菜种质幼苗期的耐铝毒特性.通过这些方法综合评价油菜对铝的耐受性,可以避免单一指标的片面性和不稳定性,结果更为可靠.In order to reduce the acidification of farmland caused by excessive application of ammonium fertilizer and frequent acid rain,which affects crop growth,a comprehensive evaluation method was used to screen aluminum tolerant germplasm through root phenotype during the seedling stage.The parents 10D130 and Zhongshuang 11 of the recombinant inbred lines(RIL)of Brassica napus were used as materials to screen the suitable aluminum stress concentrations during the seedling stage.138 seedlings of Brassica napus RIL population were treated with this concentration of aluminum solution.The coefficient of variation analysis,correlation analysis,principal component analysis,systematic cluster analysis and stepwise regression analysis were carried out for analyzing the single tolerance coefficients of aluminum toxicity of 7 root parameters,including root length,surface area,average diameter,root volume,root crossings,root tips and dry weight.The aluminum tolerance characteristics of roots in different rape lines were comprehensively evaluated by using the membership function method.The results showed that there were significant differences in root indexes among 138 rape lines(p<0.05).The aluminum-tolerant lines 27034,27036 and 27007 at seedling stage were screened out.According to the results of grey correlation degree and stepwise regression analysis,it was considered that seven key indicators such as root length,surface area,root volume,root crossings,etc.,could preliminarily determine the aluminum tolerance of Brassica napus germplasm at seedling stage.The comprehensive evaluation of the tolerance rape to aluminum by these methods can avoid the one-sidedness and instability of a single index,and the results are more reliable.

关 键 词：甘蓝型油菜 重组自交系 耐铝性 苗期 根系 

分 类 号：S634.3[农业科学—蔬菜学]