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作 者:刘宏宇 杨广 LIU Hongyu;YANG Guang(College of Fisheries,Tianjin Agricultural University,Tianjin Key Laboratory of Aquatic Ecology and Aquaculture,Tianjin 300384,China)
机构地区:[1]天津农学院水产学院,天津市水产生态及养殖重点实验室,天津300384
出 处:《黑龙江水产》2024年第2期133-139,共7页Northern Chinese Fisheries
基 金:天津市淡水养殖产业技术体系创新团队“营养需求与饲料岗位项目(编号ITTFRS2021000-011)”;天津市技术体系创新团队项目“淡水养殖产业营养需求与饲料(编号ITTFRS2021000-011)”。
摘 要:为了解水环境温度对戊二醛(glutaraldehyde,GA)杀菌效果的影响,在20℃、25℃和30℃三个温度条件下,定期检测了2株细菌(副溶血弧菌Vibrio parahaemolyticus、维氏气单胞菌Aeromonas veroni)在不同浓度戊二醛水溶液中的致死情况。检测结果显示,戊二醛对维氏气单胞菌的杀菌率与温度正相关,低药物浓度时(0.45 mg/L),30℃水体中的杀菌率在24 h达到90%以上,而较低温度(20℃)实现90%杀菌率需要27 h;当浓度升至1.8 mg/L时,30℃、5 h杀菌率可以达到90%,20℃时则需要延长至7 h;当更高药物浓度(18 mg/L)时,30℃、20 min可实现90%以上杀菌率,20℃时所需时间相应延长至30 min。戊二醛对副溶血弧菌灭菌效果的影响也呈现相似规律性,当药物浓度0.18 mg/L时,杀菌率分别在15 h(30℃)和18 h(20℃)达到90%以上;当药物浓度1.8 mg/L和18 mg/L时,30℃水体中分别于100 min和3 min达到90%以上的杀菌率;20℃时时间延长至180 min和5 min。该研究结果可为科学合理使用戊二醛提供参考。In order to research the effect of water ambient temperature on the bactericidal rate(BR)of glutaraldehyde(GA),Vibrio parahaemolyticus and Aeromonas veronii were regularly tested for mortality under three temperature conditions of 20℃,25℃,and 30℃.The results showed that:For Aeromonas veronii,the bactericidal rate was positively correlated with temperature.At low drug concentrations(0.45 mg/L),the BR reached over 90%at 30℃for 24 hours,while at low temperatures(20℃),it took 27 hours.As for drug concentrations of 1.8 mg/L,at 30℃,the BR reached 90%in 5 hours,while at 20℃,it extended to 7 hours.At higher drug concentrations(18 mg/L),it took 20 minute which the BR reached over 90%at 30℃.The time was correspondingly extended to 30 minutes when the temperature drops to 20℃.For Vibrio parahaemolyticus,a similar rule was observed.The BR reached over 90%at 15 hours(30℃)and 18 hours(20℃)when the concentration was 0.18 mg/L.At the concentrations of 1.8 mg/L and 18 mg/L,it took 100 minutes and 3 minutes that the BR reached over 90%at 30℃.When the temperature reduced to 20℃,the time was correspondingly extended to more than180 minutes and 5 minutes.This research results provide reference for the scientific and rational use of glutaraldehyde.
关 键 词:温度 戊二醛(glutaraldehyde GA) 维氏气单胞菌(Aeromonas veroni) 副溶血弧菌(Vibrio Parahaemolyticus) 杀菌率
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