不同水平的小球藻对草鱼免疫力功能的影响  

Effects of different levels of Chlorella vulgaris on the immune function of Ctenopharyngodon idellus

作　　者：闻祖银 WEN Zuyin(Qinzhou City Qinnan District Huangyutun Town Agricultural and Rural Service Center,Qinzhou 535033,Guangxi China)

机构地区：[1]广西钦州市钦南区黄屋屯镇农业农村服务中心,广西钦州535033

出　　处：《黑龙江水产》2024年第2期165-168,共4页Northern Chinese Fisheries

摘　　要：为研究不同水平的小球藻(Cholorella vulgaris)对草鱼(Ctenopharyngodon idella)机体非特异性免疫功能的影响,确定小球藻在改善草鱼机体免疫功能方面的最适添加水平。试验选择体重约20g、健康无病的草鱼400尾,按照单因素试验设计将其均分4个处理组,每组4个重复,每个重复25尾草鱼。各组草鱼依次投喂含有0%(对照组)、0.5%、1.0%、1.5%的小球藻的饲料。投喂60 d后,检测各组草鱼机体的非特异性免疫指标。检测结果显示,与对照组比较,试验2组和试验3组草鱼血清中碱性磷酸酶(ALP或AKP)活性显著提高(P<0.05),溶菌酶(LZ)活性显著提高(P<0.05),总抗氧化能力显著提高(P<0.05);超氧化物歧化酶活性显著提高(P<0.05);而小球藻对草鱼血清中的酸性磷酸酶、过氧化氢酶活力未产生显著性影响(P>0.05)。研究结果表明,草鱼养殖生产中,添加1.0%和1.5%的小球藻可以显著增强机体的非特异性免疫力。In order to study the effects of different levels of Chlorella vulgaris on the organismal non-specific immune function of grass carp(Ctenopharyngodon idella),and to determine the optimal level of addition of Chlorella vulgaris in improving the organismal immune function of grass carp.In the experiment,400 healthy and disease-free grass carp weighing about 20g were selected and divided into 4 treatment groups with 4 replicates each and 25 fish in each replicate according to the one-way experimental design.Each group was fed with 0%(control),0.5%,1.0%and 1.5%chlorella vulgaris.After 60 d of feeding,the non-specific immunity indexes of the body of grass carp in each group were detected.The test results showed that compared with the control group,alkaline phosphatase(ALP or AKP)activity in the serum of grass carp in the test 2 and test 3 groups was significantly increased(P<0.05),lysozyme(LZ)activity was significantly increased(P<0.05),total antioxidant capacity was significantly increased(P<0.05);and superoxide dismutase activity was significantly increased(P<0.05);and the effect of Chlorella on the serum of grass carp was significantly increased(P<0.05).The acid phosphatase and catalase activities in grass carp serum were not significantly affected(P>0.05).The results of this study indicated that the addition of 1.0%and 1.5%chlorella vulgaris could significantly enhance the non-specific immunity of grass carp in aquaculture production.

关 键 词：小球藻(Cholorella vulgaris) 草鱼(Ctenopharyngodon idella) 溶菌酶(LZ) 碱性磷酸酶(ALP或AKP) 超氧化物歧化酶(SOD) 

分 类 号：S965.112[农业科学—水产养殖]