盐胁迫对平欧杂种榛根系生理生化特性的影响  

Effects of Salt Stress on the Physiological and Biochemical Characteristics of Ping’ou Hybrid Hazelnut Root Systems

作　　者：罗达 宋锋惠[2] 卢明艳[2] 史彦江[2] Luo Da;Song Fenghui;Lu Mingyan;Shi Yanjiang(Huaihua University,Huaihua 418008,P.R.China;Institute of Economic Forests,Xinjiang Academy of Forestry Science)

机构地区：[1]怀化学院,怀化418008 [2]新疆林业科学院经济林研究所

出　　处：《东北林业大学学报》2024年第4期29-33,45,共6页Journal of Northeast Forestry University

基　　金：新疆维吾尔自治区自然科学基金项目(2022D01A62);国家自然科学基金项目(31960324);新疆“天山英才”计划(TSYC-343)。

摘　　要：以2年生平欧杂种榛‘达维’为试验材料,在水培条件下设置对照、轻度、中度和重度盐胁迫处理(NaCl浓度分别为0、50、100和200 mmol·L^(-1)),研究不同胁迫时间(1、3和7 d)下根系渗透调节物质、膜伤害程度、抗氧化酶活性以及离子特征的变化,探讨耐盐的生理机制。结果表明:盐胁迫显著促进了可溶性糖(SS)和脯氨酸(Pro)的积累。SS随着胁迫时间的延长呈持续增加趋势,Pro在重度胁迫至3 d时显著升高,较胁迫1 d时提高25.9%。相对电导率(REC)和丙二醛(MDA)随着盐胁迫程度的增强均呈不断升高,重度胁迫下二者较对照分别显著提高32.1%和55.6%。REC和MDA随着胁迫时间的延长呈不断升高。随着盐胁迫程度的增强,超氧化物歧化酶(SOD)表现为先下降后上升,过氧化氢酶(CAT)呈不断增加。SOD和CAT对盐胁迫的响应存在时间差异,二者分别在胁迫7和1 d时达到最大值。随着盐胁迫程度的增强,Na^(+)不断升高,K^(+)波动下降,w(K^(+))∶w(Na^(+))表现为不断下降。平欧杂种榛具有一定的耐盐性。轻度胁迫下,幼苗可以通过自身生理生化特性的调节以响应和适应盐胁迫;中度胁迫下,根细胞膜开始受到伤害,但仍能通过积累渗透调节物质和增强抗氧化酶活性来应对盐胁迫;当NaCl浓度达到200 mmol·L^(-1)且胁迫超过3 d时,植物开始遭受盐害。SS、Pro、CAT活性和Na^(+)质量分数可作为平欧杂种榛耐盐性评价的参考指标。Using 2-year-old ping’ou hybrid hazelnut‘Dawei’as the experimental material,control,mild,moderate,and severe salt stress treatments(NaCl concentrations of 0,50,100,and 200 mmol·L^(-1))were set under hydroponic conditions to study the changes in root osmotic adjustment substances,membrane damage degree,antioxidant enzyme activity,and ion characteristics at different stress times(1,3,and 7 days),and explore the physiological mechanism of salt tolerance.The results showed that salt stress significantly promoted the accumulation of soluble sugars(SS)and proline(Pro).SS showed a continuous increasing trend with the prolongation of stress time,while Pro significantly increased at severe stress to 3 days,with a 25.9%increase compared to 1 day of stress.Relative electrical conductivity(REC)and malondialdehyde(MDA)increased continuously with the enhancement of salt stress,with a significant increase of 32.1%and 55.6%under severe stress compared to the control.REC and MDA increased continuously with the prolongation of stress time.With the enhancement of salt stress,superoxide dismutase(SOD)first decreased and then increased,while catalase(CAT)showed a continuous increase.SOD and CAT responded to salt stress with time differences,reaching their maximum values at 7 and 1 day of stress,respectively.With the enhancement of salt stress,Na+increased continuously,K+fluctuated and decreased,and w(K^(+))∶w(Na^(+))decreased continuously.The ping’ou hybrid hazelnut‘Dawei’exhibited a certain degree of salt tolerance.Under mild stress,seedlings could respond and adapt to salt stress through the regulation of their own physiological and biochemical characteristics.Under moderate stress,root cell membranes began to be damaged,but plants could still cope with salt stress by accumulating osmotic adjustment substances and enhancing antioxidant enzyme activity.When the NaCl concentration reached 200 mmol·L^(-1) and the stress exceeded 3 days,plants began to suffer from salt damage.SS,Pro,CAT activity,and Na+content can be us

关 键 词：平欧杂种榛 NACL胁迫 根 生理生化特性 耐盐性 

分 类 号：Q945.78[生物学—植物学]