金纳米向日葵用于免标记SERS检测电刺激细胞凋亡过程中DNA损伤  

Label-free SERS Srategy for Detection of DNA Base Damage in Stimulus-Induced Cell Apoptosis by Plasmonic SERS Au Nanosunflowers

作　　者：齐国华 金永东 QI Guohua;JIN Yongdong(State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,Jilin,P.R.China;School of Biomedical Engineering,Shenzhen University,Shenzhen 518060,Guangdong,P.R.China)

机构地区：[1]中国科学院长春应用化学研究所电分析化学国家重点实验室,吉林省长春市130012 [2]深圳大学生物医学工程学院,广东省深圳市518060

出　　处：《光散射学报》2024年第1期38-43,共6页The Journal of Light Scattering

基　　金：国家自然科学基金青年基金项目(22004117);中国科学院特别研究助理择优资助项目支持。

摘　　要：精准的诊断以及分析癌细胞死亡过程中复杂基因组DNA损伤,对肿瘤的治疗至关重要,但是仍然面临着重要的挑战。本研究设计了尺寸均一的等离子体金纳米向日葵,该纳米结构主要是通过修饰多肽的金纳米粒子静电组装的方法制备,形成热点结构丰富的SERS基底,用于捕获基因组DNA,实现高灵敏度基因组中DNA的SERS检测。我们的研究发现在恒压1.2 V刺激5 min的条件下,癌细胞比正常细胞更容易死亡。通过SERS光谱分析,我们可以看出,在电刺激癌细胞凋亡过程中,基因组中DNA的双链断裂,且腺嘌呤的缺失,这些加速了癌细胞的死亡过程,进而影响DNA的复制和转录。我们开发的传感平台对研究基因相关的疾病提供了重要的应用价值。Molecular profiling and accurate damage analysis of complex biomolecular events in tumor cells are critical to the diagnosis and treatment of cancer.However,due to sensitivity limitation and dynamic and complex nature of apoptosis process,label-free detection of cellular DNA damage during cancer therapeutic treatment,at the DNA bases level,is still a huge challenge.Herein,by designed preparation of novel and uniform plasmonic sunflower-like assembly gold(Au)nanostructure that capable of efficient DNA capture and providing high-density“hot spots”for SERS enhancement,we succeeded in sensitive and reliable SERS detection of DNA damage in apoptotic cancer cells at the DNA bases level.The chemical structure damage of cellular DNA caused by electrostimulus-induced cell apoptosis was revealed and discriminated at the bases level,for the first time,by label-free SERS detection with the nano-sunflowers.The SERS results showed that the external electrostimulus(at 1.2 V,for 5 min)was almost harmless to normal healthy cells but it caused pronounced double strands break and Adenine(A)base damage in cancer cell DNAs,which effectively destroyed the reproduction and transcribe of DNA to harass cancer cell mitosis and ultimately induce cell apoptosis.The finding provides deep insights into molecular genomic DNA damages of cancer cells during theranostic process,and the method would open a new avenue for the study of genetically related diseases.

关 键 词：表面增强拉曼光谱 电刺激 基因组DNA 细胞凋亡 

分 类 号：O657.37[理学—分析化学] Q291[理学—化学]